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Comparison of protein solubilization methods suitable for proteomic analysis of soybean seed proteins.
TLDR
The results suggest that the thiourea/urea and TCA methods are efficient and reliable methods for 2D separation of soybean seed proteins and subsequent identification by mass spectrometry. Expand
Proteomic analyses of male contributions to honey bee sperm storage and mating
TLDR
Of the 69 unique honey bee proteins found, 66 are also in Drosophila melanogaster, and there is over‐representation of genes implicated in the glycolysis pathway. Expand
Interrelationships between sex and exogenous growth hormone administration on performance, body composition and protein and fat accretion of growing pigs.
TLDR
Pigs used in this study and treated with pGH exhibited a rate of protein deposition far greater than previously reported, and as such redefine the genetic capacity for lean tissue growth by the pig. Expand
Effect of dietary betaine on nutrient utilization and partitioning in the young growing feed-restricted pig.
TLDR
The data suggest that betaine alters nutrient partitioning such that carcass protein deposition is enhanced at the expense of carcass fat and in part, visceral tissue. Expand
Bovine Blastocyst-Derived Trophectoderm and Endoderm Cell Cultures: Interferon Tau and Transferrin Expression as Respective In Vitro Markers
TLDR
This simple coculture method for the in vitro propagation of bovine trophectoderm and endoderm provides a system for assessing their biology in vitro. Expand
Characterization of storage proteins in wild (Glycine soja) and cultivated (Glycine max) soybean seeds using proteomic analysis.
TLDR
Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) with three different immobilized pH gradient (IPG) strips was an effective method to separate a large number of abundant and less-abundant storage proteins. Expand
Critical control points in the impact of the proinflammatory immune response on growth and metabolism.
TLDR
Attenuation of these overt posttranslational protein modifications at their site of production offers a strategy to minimize their detrimental impact while preserving needed cytokine, NO, and O2(.-) functions. Expand
Impact of solar ultraviolet-B on the proteome in soybean lines differing in flavonoid contents.
TLDR
The results suggest that high levels of flavonoids lead to a reduction in UV-B sensitivity at the proteomic level, and differentially accumulated spots were identified by mass spectrometry. Expand
Separation and identification of soybean leaf proteins by two-dimensional gel electrophoresis and mass spectrometry.
TLDR
The results indicate that 2D-PAGE, combined with MALDI-TOF-MS and LC-MS/MS, is a sensitive and powerful technique for separation and identification of soybean leaf proteins. Expand
Identification of protein carbonyls in serum of the fetal and neonatal pig.
TLDR
While abundance and distribution of oxidized proteins changed over time, these changes appear to primarily reflect relative amounts of those proteins in serum. Expand
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