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The new plant virus family Flexiviridae and assessment of molecular criteria for species demarcation
The new plant virus family Flexiviridae is described. The family is named because its members have flexuous virions and it includes the existing genera Allexivirus, Capillovirus, Carlavirus,
The family Closteroviridae revised
The mealybug-transmitted species have been separated from the genus Closterovirus and accommodated in a new genus named Ampelovirus (from ampelos, Greek for grapevine), and the family now comprises three genera.
A polymerase chain reaction assay adapted to plum pox potyvirus detection.
A sensitive, polyvalent assay based on the polymerase chain reaction (PCR) was developed for plum pox potyvirus (PPV) detection and proved to be more sensitive than molecular hybridization using 32P-labelled RNA probes for PPV detection.
Top 10 plant viruses in molecular plant pathology.
A short review on each virus of the Top 10 list and its importance is presented, with the intent of initiating discussion and debate amongst the plant virology community, as well as laying down a benchmark, as it will be interesting to see in future years how perceptions change and which viruses enter and leave the Top10.
A highly sensitive immunocapture polymerase chain reaction method for plum pox potyvirus detection.
A highly sensitive assay, based on polymerase chain reaction amplification of cDNA synthesized from the viral RNA of antibody-captured viral particles, has been developed for plum pox potyvirus (PPV)
Nucleotide sequence and genomic organization of apple chlorotic leaf spot closterovirus.
Results show that ACLSV and probably at least the subgroup A of closteroviruses should be regarded as members of the "Sindbis-like" supergroup of RNA viruses.
Geographically and temporally distant natural recombinant isolates of Plum pox virus (PPV) are genetically very similar and form a unique PPV subgroup.
Results indicate that recombinant isolates represent an evolutionarily successful, homogeneous group of isolate with a common history and unique founding recombination event, and the name PPV-Rec is proposed for this coherent ensemble of isolates.
Frequent occurrence of recombinant potyvirus isolates.
Recombination was detected or suspected in 18 out of 109 potyvirus isolates tested, belonging to four out of eight virus species, and was most prevalent in potato virus Y, clear in bean common mosaic virus, and possible in bean yellow mosaic and zucchini yellow mosaic viruses.
The Eukaryotic Translation Initiation Factor 4E Controls Lettuce Susceptibility to the Potyvirus Lettuce mosaic virus1
The eIF4E and eIF(iso)4E cDNAs from several genotypes of lettuce (Lactuca sativa) that are susceptible, tolerant, or resistant to infection by Lettuce mosaic virus (LMV; genus Potyvirus) were cloned