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Genome Sequences of Three Agrobacterium Biovars Help Elucidate the Evolution of Multichromosome Genomes in Bacteria
Together, these results define the evolution of chromosome architecture and gene content among the Rhizobiaceae and support a generalized mechanism for second-chromosome formation among bacteria. Expand
Upstream Migration of Xylella fastidiosa via Pilus-Driven Twitching Motility
It is demonstrated that two knockout mutants that are deficient in type IV pili do not twitch and are inhibited from colonizing upstream vascular regions in planta, and mutants with insertions in pilB or pilQ express enhanced biofilm formation, whereas a mutant with an insertion in fimA is biofilm deficient. Expand
Transformation of five grape rootstocks with plant virus genes and a virE2 gene from Agrobacterium tumefaciens
To facilitate the development of transgenic grapevines that are resistant to grapevine fanleaf virus, grapevine leafroll-associated closterovirus and crown gall diseases, a rapid system for regenerating root-stocks was developed. Expand
Type I and type IV pili of Xylella fastidiosa affect twitching motility, biofilm formation and cell-cell aggregation.
Xylella fastidiosa, an important phytopathogenic bacterium, causes serious plant diseases including Pierce's disease of grapevine. It is reported here that type I and type IV pili of X. fastidiosaExpand
CROWN GALL OF GRAPE: Biology and Disease Management.
Control practices rely on the use of disease-resistant cultivars, cultural practices that minimize plant injury, and the production of pathogen-free vines, and promising future controls include employment of biological control agents and development of crown gall-resistant transgenic grapevines. Expand
Assessing Adhesion Forces of Type I and Type IV Pili of Xylella fastidiosa Bacteria by Use of a Microfluidic Flow Chamber
The experimental results demonstrate that microfluidic flow chambers are useful and convenient tools for assessing the drag forces necessary for detaching bacterial cells and that with specific pilus mutants, the role of the pilus type can be further assessed. Expand
Homologous Streptomycin Resistance Gene Present among Diverse Gram-Negative Bacteria in New York State Apple Orchards
The streptomycin resistance gene of Pseudomonas syringae pv. papulans Psp36 was cloned into Escherichia coli and used to develop a 500-bp DNA probe that is specific for streptomycin resistance in P.Expand
A luxR homolog, aviR, in Agrobacterium vitis is associated with induction of necrosis on grape and a hypersensitive response on tobacco.
R reverse transcriptase-polymerase chain reaction analysis indicated that ORF2 and ORF14 may be regulated by an aviR-encoded transcriptional regulator. Expand
Novel pathogen-specific primers for the detection of "Agrobacterium vitis" and "Agrobacterium tumefaciens"
A wide range of agrob bacteria occurring on grapevine were detected and identified and results confirm that vitopine-type agrobacteria form a distinct group within the genus Agrobacterium. Expand