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ITS primers with enhanced specificity for basidiomycetes ‐ application to the identification of mycorrhizae and rusts
TLDR
ITS1‐F/ITS4‐B preferential amplification was shown to be particularly useful for detection and analysis of the basidiomycete component in ectomycorrhizae and in rust‐infected tissues. Expand
Towards a unified paradigm for sequence‐based identification of fungi
TLDR
All fungal species represented by at least two ITS sequences in the international nucleotide sequence databases are now given a unique, stable name of the accession number type, and the term ‘species hypothesis’ (SH) is introduced for the taxa discovered in clustering on different similarity thresholds. Expand
Specific amplification of 18S fungal ribosomal genes from vesicular-arbuscular endomycorrhizal fungi colonizing roots.
TLDR
A polymerase chain reaction primer (VANS1) is developed that enables the specific amplification of a portion of the vesicular-arbuscular endomycorrhizal fungus small subunit rRNA directly from a mixture of plant and fungal tissues. Expand
The molecular revolution in ectomycorrhizal ecology: peeking into the black‐box
TLDR
Results from these studies have revealed that EM communities are impressively diverse and are patchily distributed at a fine scale below ground, but there is a poor correspondence between fungi that appear dominant as sporocarps vs. those that seem dominant on roots. Expand
Community structure of ectomycorrhizal fungi in a Pinus muricata forest: above- and below-ground views
TLDR
By using molecular methods based on polymerase chain reaction, this work was able to identify the fungal symbionts directly from mycorrhizae in nearly all of the myCorrhizal morphotypes the authors found. Expand
Changing partners in the dark: isotopic and molecular evidence of ectomycorrhizal liaisons between forest orchids and trees
TLDR
These findings represent a major shift in the understanding of both orchid ecology and evolution because they explain how orchids can thrive in low–irradiance niches and they show that a shift to exploiting ectomycorrhizal fungi precedes viable losses of photosynthetic ability in orchid lineages. Expand
Mycorrhizal colonization of Pinus muricata from resistant propagules after a stand-replacing wildfire
TLDR
Fire appeared to have either stimulated or provided a competitive advantage to R. olivaceotinctus, which increased in abundance on the post-fireBioassay and field seedlings, and the number and frequency of mycorrhizal taxa on bioassay seedlings decreased with increased dilution. Expand
Indoor fungal composition is geographically patterned and more diverse in temperate zones than in the tropics
TLDR
The results suggest that factors driving fungal composition are primarily global rather than mediated by building design or function, and it is shown that fungal diversity is significantly higher in temperate zones than in the tropics, with distance from the equator being the best predictor of phylogenetic community similarity. Expand
Fungal Molecular Systematics
TLDR
The fungi, as thus defined, are of great importance for the following reasons: (a) They are the primary decomposers in all terrestrial ecosystems; (b) they are important symbiotic associates of vascular plants both in mutualistic and parasitic relationships. Expand
Community structure of ectomycorrhizal fungi in a Pinus muricata forest: minimal overlap between the mature forest and resistant propagule communities
TLDR
It is suggested that species differences in resource preferences and colonization strategies, such as those documented here, contribute to the maintenance of species richness in the ectomycorrhizal community. Expand
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