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Effects of 17alpha-ethinylestradiol in a fathead minnow (Pimephales promelas) gonadal recrudescence assay.
Exposure to EE2 resulted in a significant decrease in GSI, condition factor, and number of batches of eggs and their fertilization rate at EE2 exposure concentrations between 10 and 100 ng/L, with a dose-dependent decrease in the mean number of eggs per pair. Expand
Is the fish embryo toxicity test (FET) with the zebrafish (Danio rerio) a potential alternative for the fish acute toxicity test?
Results confirm that fish embryo tests are neither better nor worse than acute fish toxicity tests and provide strong scientific support for the FET as a surrogate for the acute fishoxicity test. Expand
Towards an alternative for the acute fish LC(50) test in chemical assessment: the fish embryo toxicity test goes multi-species -- an update.
Results document that the transfer of the protocol is possible with only minor modifications and examples are given as to how additional endpoints can be incorporated into the fish embryo test protocol to extend its scope, e.g. to sediment toxicity assessment or genotoxicity and mutagenicity testing. Expand
The use of Fish Cells in Ecotoxicology: The Report and Recommendations of ECVAM Workshop 471, 2
This is the report of the forty-seventh of a series of workshops organised by the European Centre for the Validation of Alternative Methods (ECVAM), held in Angera, Italy, on 22–24 October 2001, under the co-chairmanship of Argelia Castaño and Helmut Segner. Expand
Dechorionation as a tool to improve the fish embryo toxicity test (FET) with the zebrafish (Danio rerio).
With appropriate modifications of the fish embryo test protocol, embryos can be dechorionated at 24h post-fertilization (hpf) with survival rates of ≥90%. Expand
A new sediment contact assay to assess particle-bound pollutants using zebrafish (danio rerio) embryos
Goal, Scope and Background. Based on a bioassay battery covering only primary producers and consumers as well as degraders, the potential ecological hazard of sediments to vertebrates cannot beExpand
Effects of 17a-ethinylestradiol on the expression of three estrogen-responsive genes and cellular ultrastructure of liver and testes in male zebrafish.
In sexually differentiated males no reorganisation of the gonadal tissue towards an ovary could be definitely detected at any level investigated; rather, destructive phenomena like phagocytosis of sperm cells by Sertoli cells were observed. Expand
Sediment genotoxicity in the Tietê River (São Paulo, Brazil): in vitro comet assay versus in situ micronucleus assay studies.
Either in vitro comet assay or in situ micronucleus assay was successful in identifying site-specific differences in genotoxicity, with a high correlation between in situ and in vitro results indicating the relevance of the latter even for environmental studies. Expand
Ecotoxicological Assessment of Sediment, Suspended Matter and Water Samples in the Upper Danube River. A pilot study in search for the causes for the decline of fish catches (12 pp)
The complex pattern of ecotoxicological effects caused by environmental samples from the Danube River, when assessed in an in vitro biotest battery using both acute and more specific endpoints, showed that integration of different endpoints is essential for appropriate hazard assessment. Expand
Development of a flow-through system for the fish embryo toxicity test (FET) with the zebrafish (Danio rerio).
A flow-through system for the FET using modified commercially available polystyrene 24-well microtiter plates was developed, thus combining the advantages of the standard FET with those of continuous delivery of test substances, offering the possibility for teratogenicity testing. Expand