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Antibiotic resistance of bacterial biofilms.
Biofilms can be prevented by early aggressive antibiotic prophylaxis or therapy and they can be treated by chronic suppressive therapy and a promising strategy may be the use of enzymes that can dissolve the biofilm matrix as well as quorum-sensing inhibitors that increase biofilm susceptibility to antibiotics. Expand
Screening for quorum-sensing inhibitors (QSI) by use of a novel genetic system, the QSI selector.
GeneChip-based transcriptome analysis revealed that garlic extract and 4-NPO had specificity for QS-controlled virulence genes in Pseudomonas aeruginosa, and these two QSIs significantly reduced P. aerug inosa biofilm tolerance to tobramycin treatment as well as virulence in a Caenorhabditis elegans pathogenesis model. Expand
Pseudomonas aeruginosa biofilms in cystic fibrosis.
The persistence of chronic Pseudomonas aeruginosa lung infections in cystic fibrosis patients is due to biofilm-growing mucoid (alginate-producing) strains, which are the major cause of the lung tissue damage in CF. Expand
Pseudomonas aeruginosa biofilms in the respiratory tract of cystic fibrosis patients
Investigation of the appearance and location of Pseudomonas aeruginosa in the cystic fibrosis lung and in sputum finds preserved tissues of CF patients who died due to chronic P. aerugInosa lung infection and routine sputums from 77 chronically P. Aerug inosa infected CF patients. Expand
Identity and effects of quorum-sensing inhibitors produced by Penicillium species.
Quorum sensing (QS) communication systems are thought to afford bacteria with a mechanism to strategically cause disease. One example is Pseudomonas aeruginosa, which infects immunocompromisedExpand
Why chronic wounds will not heal: a novel hypothesis
It is proposed that the lack of proper wound healing is at least in part caused by inefficient eradication of infecting, opportunistic pathogens, a situation reminiscent of chronic Pseudomonas aeruginosa infections found in patients suffering from cystic fibrosis (CF). Expand
The clinical impact of bacterial biofilms
Bacterial biofilms are resistant to antibiotics, disinfectant chemicals and to phagocytosis and other components of the innate and adaptive inflammatory defense system of the body and they can be treated by chronic suppressive antibiotic therapy. Expand
The in vivo biofilm.
Why the current in vitro models of biofilms might be limited for describing infectious biofilmms are discussed, and new strategies for improving this discrepancy are suggested. Expand
Pseudomonas aeruginosa tolerance to tobramycin, hydrogen peroxide and polymorphonuclear leukocytes is quorum-sensing dependent.
It is demonstrated that biofilm bacteria in which QS is blocked either by mutation or by administration of QS inhibitory drugs are sensitive to treatment with tobramycin and H2O2, and are readily phagocytosed by PMNs, in contrast to bacteria with functional QS systems. Expand
Effects of Antibiotics on Quorum Sensing in Pseudomonas aeruginosa
Results from the virulence factor assays, reverse transcription-PCR, and the DNA microarrays support the finding that AZM, CFT, and CPR decrease the expression of a range of QS-regulated virulence factors, suggesting the underlying mechanism may be mediated by changes in membrane permeability. Expand