Primer3Plus, an enhanced web interface to Primer3
- A. Untergasser, H. Nijveen, X. Rao, T. Bisseling, R. Geurts, J. Leunissen
- Computer ScienceNucleic Acids Res.
- 7 May 2007
A new web interface to the popular Primer3 primer design program, developed in close collaboration with molecular biologists and technicians regularly designing primers, that provides an intuitive user interface using present-day web technologies and allows easy expansion or integration of external software packages.
The Medicago Genome Provides Insight into the Evolution of Rhizobial Symbioses
- N. Young, F. Debellé, B. Roe
- 18 October 2011
The draft sequence of the M. truncatula genome sequence is described, a close relative of alfalfa (Medicago sativa), a widely cultivated crop with limited genomics tools and complex autotetraploid genetics, which provides significant opportunities to expand al falfa’s genomic toolbox.
LysM Domain Receptor Kinases Regulating Rhizobial Nod Factor-Induced Infection
- E. Limpens, Carolien Franken, P. Smit, J. Willemse, T. Bisseling, R. Geurts
- 24 October 2003
Using reverse genetics in M. truncatula, it is shown that two LYK genes are specifically involved in infection thread formation, which strongly suggests that they are Nod factor entry receptors.
NSP1 of the GRAS Protein Family Is Essential for Rhizobial Nod Factor-Induced Transcription
- P. Smit, John Raedts, R. Geurts
- 17 June 2005
The identification of the Medicago GRAS-type protein Nodulation signaling pathway 1 (NSP1), which is essential for all known Nod factor–induced changes in gene expression, is reported.
A Putative Ca2+ and Calmodulin-Dependent Protein Kinase Required for Bacterial and Fungal Symbioses
- J. Levy, C. Bres, F. Debellé
- 27 February 2004
DMI3, a Medicago truncatula gene that acts immediately downstream of calcium spiking in this signaling pathway and is required for both nodulation and mycorrhizal infection, has high sequence similarity to genes encoding calcium and calmodulin-dependent protein kinases (CCaMKs).
RNA interference in Agrobacterium rhizogenes-transformed roots of Arabidopsis and Medicago truncatula.
- E. Limpens, J. Ramos, R. Geurts
- Environmental ScienceJournal of Experimental Botany
- 1 May 2004
Evidence is presented which shows that RNAi is cell autonomous in the root epidermis, and it is shown that RNA silencing does not spread systemically to non-co-transformed (lateral) roots and only inefficiently to the non-transgenic shoot.
The role of actin in root hair morphogenesis: studies with lipochito-oligosaccharide as a growth stimulator and cytochalasin as an actin perturbing drug
- D. Miller, N. Ruijter, T. Bisseling, A. Emons
It is concluded that elongating net-axial fine bundles of actin filaments, which are called FB-actin, function in polar growth by targeting and releasing Golgi vesicles to the vesicle-rich region, while actin filament bundles looping through the tip impede vESicle retention.
Symbiotic Nitrogen Fixation.
- P. Mylona, K. Pawlowski, T. Bisseling
- BiologyThe Plant Cell
- 1 July 1995
This review describes the early steps of the interaction between rhizobia and legumes that result in the formation of a nitrogen-fixing nodule and focuses on the role of specific lipooligosaccharides secreted by Rhizobia in the induction of these early steps.
Single Nucleus Genome Sequencing Reveals High Similarity among Nuclei of an Endomycorrhizal Fungus
- Kui Lin, E. Limpens, Sanwen Huang
- BiologyPLoS Genetics
- 1 January 2014
It is demonstrated that Glomeromycota are more closely related to Mucoromycotina than to its postulated sister Dikarya, and demystifies a long-lasting hypothesis on the complex genetic makeup of arbuscular endomycorrhizal fungi.
A Sequence-Based Genetic Map of Medicago truncatula and Comparison of Marker Colinearity with M. sativa
- Hong-Kyu Choi, Dongjin Kim, D. Cook
- 1 March 2004
A core genetic map of the legume Medicago truncatula has been established by analyzing the segregation of 288 sequence-characterized genetic markers in an F2 population composed of 93 individuals, indicating that the two Medicago genomes are highly similar and establishes the basis for a Medicago composite map.