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Preliminary characterisation of a lipolytic activity from an extremely halophilic archaeon, Natronococcus sp.
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Lipolytic activity from Halobacteria: screening and hydrolase production.
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A new species of Pythium isolated from the Burgundy region in France.
TLDR
Morphological and reproductive aspects of Pythium nodosum as well as the PCR of the internal transcribed spacer (ITS1) of the ribosomal nuclear DNA coupled with restriction fragment length polymorphism analysis are described here.
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Phenotypic and genotypic characterization of rhizobia associated with Acacia saligna (Labill.) Wendl. in nurseries from Algeria.
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Diversity of Paenibacillus polymyxa populations in the rhizosphereof wheat (Triticum durum) in Algerian soils
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Directed Evolution of the Epoxide Hydrolase from Aspergillus niger
TLDR
An efficient and genetically stable expression system for the directed evolution of epoxide hydrolase from Aspergillus niger (ANEH) has been constructed and a screening assay for determining the enantioselectivity in the kinetic resolution of styrene oxide has been established using mass spectrometry.
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Non-Aqueous Biocatalysis in Heterogeneous Solvent Systems
Nadia Krieger1*, Tej Bhatnagar2, Jacques C. Baratti2, Alessandra M. Baron1, Valéria M. de Lima1 and David Mitchell3 Biocatalysis and Enzyme Technology Laboratory, Applied Chemistry Research Centre…
Colorimetric Assays for Quantitative Analysis and Screening of Epoxide Hydrolase Activity
TLDR
Two colorimetric assays have been optimized to characterize variants issued from the directed evolution of the EH from Aspergillus niger, and are sufficiently reliable for quantitative screening of EH activity in microtiter plates and are low cost alternatives to GC or MS analysis.
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A spectrophotometric method to assay epoxide hydrolase activity.
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H107, a new aminoglycoside anti-Pseudomonas antibiotic produced by a new strain of Spirillospora.
TLDR
This is the first report of aminoglycoside anti-Pseudomonas production by Spirillospora, which was obtained only from the broth filtrate after precipitation with acetic acid followed by extraction with n-butanol.
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