Phosphoinositides: tiny lipids with giant impact on cell regulation.
- T. Balla
- BiologyPhysiological Reviews
- 1 July 2013
This review is an attempt to give an overview of this enormous research field focusing on major developments in diverse areas of basic science linked to cellular physiology and disease.
Chaperone-mediated coupling of endoplasmic reticulum and mitochondrial Ca2+ channels
It is demonstrated that VDAC1 is physically linked to the endoplasmic reticulum Ca2+-release channel inositol 1,4,5-trisphosphate receptor (IP3R) through the molecular chaperone glucose-regulated protein 75 (grp75) and functional interaction between the channels was shown by the recombinant expression of the ligand-binding domain of the IP3R on the ER or mitochondrial surface.
Structural and functional features and significance of the physical linkage between ER and mitochondria
An unexpected dependence of cell function and survival is revealed on the maintenance of proper spacing between the ER and mitochondria, which makes mitochondria prone to Ca2+ overloading and ensuing permeability transition.
Visualization of Phosphoinositides That Bind Pleckstrin Homology Domains: Calcium- and Agonist-induced Dynamic Changes and Relationship to Myo-[3H]inositol-labeled Phosphoinositide Pools
Identification and dynamic imaging of phosphoinositides that interact with PH domains will further the understanding of the regulation of such proteins by inositol phospholipids.
Viral Reorganization of the Secretory Pathway Generates Distinct Organelles for RNA Replication
A Pharmacological Map of the PI3-K Family Defines a Role for p110α in Insulin Signaling
Phosphatidylinositol 4-kinases: old enzymes with emerging functions.
Polarization of chemoattractant receptor signaling during neutrophil chemotaxis.
- G. Servant, O. Weiner, P. Herzmark, T. Balla, J. Sedat, H. Bourne
- Biology, ChemistryScience
- 11 February 2000
Morphologic polarity is necessary for chemotaxis of mammalian cells and the pleckstrin homology domain of the AKT protein kinase, tagged with the green fluorescent protein (PHAKT-GFP), was expressed in neutrophils to probe intracellular signals responsible for this asymmetry.
Recruitment and activation of a lipid kinase by hepatitis C virus NS5A is essential for integrity of the membranous replication compartment.
Phosphatidylinositol 4,5-bisphosphate and Arf6-regulated membrane traffic
Results demonstrate that PIP 5-kinase activity and PIP2 turnover controlled by activation and inactivation of Arf6 is critical for trafficking through the Arf 6 PM-endosomal recycling pathway.