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Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection
TLDR
These mutants—the ‘Keio collection’—provide a new resource not only for systematic analyses of unknown gene functions and gene regulatory networks but also for genome‐wide testing of mutational effects in a common strain background, E. coli K‐12 BW25113. Expand
Hd1, a Major Photoperiod Sensitivity Quantitative Trait Locus in Rice, Is Closely Related to the Arabidopsis Flowering Time Gene CONSTANS
TLDR
It is suggested that Hd1 functions in the promotion of heading under short- day conditions and in inhibition under long-day conditions and is a homolog of CONSTANS in Arabidopsis. Expand
Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110
TLDR
Direct DNA sequencing of PCR products for the 251 regions with short indel and base disparities revealed that only eight sites are true differences, and errors in the original MG1655 sequence were mostly within portions sequenced with out‐dated technology based on radioactive chemistry. Expand
Genome Structure of the Legume, Lotus japonicus
TLDR
Structural features of the L. japonicus genome are reported, providing the first opportunity to look into the complex and unique genetic system of legumes. Expand
Multiple High-Throughput Analyses Monitor the Response of E. coli to Perturbations
TLDR
E. coli seems to use complementary strategies that result in a metabolic network robust against perturbations, and actively regulated enzyme levels to maintain a stable metabolic state in response to changes in growth rate. Expand
The genome sequence and structure of rice chromosome 1
TLDR
The essentially complete sequence of chromosome 1, the longest chromosome in the rice genome, is reported and characteristics of the chromosome structure and the biological insight gained from the sequence are summarized. Expand
Ribosome binding proteins YhbH and YfiA have opposite functions during 100S formation in the stationary phase of Escherichia coli
TLDR
YhbH and YfiA have opposite functions in 70S dimer formation, suggesting that their binding sites overlap and they compete for a region proximal to the P‐ and A‐sites on 30S subunits. Expand
Large-scale identification of protein-protein interaction of Escherichia coli K-12.
TLDR
An extended analysis of these interacting networks by bioinformatics and experimentation should provide new insights and novel strategies for E. coli systems biology. Expand
Responses of theCentral Metabolism in Escherichia coli to PhosphoglucoseIsomerase and Glucose-6-Phosphate DehydrogenaseKnockouts
TLDR
Although knockout of G6P dehydrogenase had no significant influence on the central metabolism under glucose-limited conditions, this mutation resulted in extensive overflow metabolism and extremely low tricarboxylic acid cycle fluxes under ammonia limitation conditions. Expand
Experimental and Computational Assessment of Conditionally Essential Genes in Escherichia coli
TLDR
An integrative model-driven analysis of the Keio E. coli mutant collection screened in this study establishes a paradigm that will drive model enhancement while simultaneously generating hypotheses that will ultimately lead to a better understanding of the organism. Expand
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