The processes of DNA replication, DNA ligation, and RNA transcription have provided the basis of distinct and separate in vitro nucleic acid amplification strategies. The first described and the most widely utilized amplification protocol is the polymerase chain reaction (PCR).O,2) The PCR method consists of multiple cycles of oligonu-cleotide… (More)
The 5'-end attachment of oligonucleotides to dextran supports facilitates the study of the hybridization properties of an immobilized oligonucleotide system. The hybridization properties which were studied include: hybridization capacity and kinetics, hybridization-complex stability, and reagents influencing hybridization efficiency. Results of these… (More)
A collection of computer programs is described which permit automatic entering of nucleotide sequence data directly from an autoradiograph into a computer. This collection, called DIGITPAD, makes use of a digitizing tablet for the data entry and allows the rapid and accurate transfer of the sequence into the computer.