T. Ogouchi

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A sandwich transfer enzyme immunoassay for elcatonin (ECT) and its usability for the pharmacokinetic study are described. The anti-salmon calcitonin (SCT) antibody was used for the present assay. The assay procedure consisted of the reaction of ECT with 2,4-dinitrophenylbiotinyl anti-SCT IgG and anti-SCT Fab'-beta-D-galactosidase conjugate, trapping onto(More)
~ . Bisphosphonates are well antirresorptive known drugs, the antiosteoclastic effect of which has been recently suggested to be brought at ]east in part through osteoblasts. Based on this, we have studied the effect of etidronate on the production of IL-I, TNF and IL-6 by peripheral blood mononuclear cells (PBMC) and MG63 cells. For comparison, two other(More)
A novel method to assess mucosal immune response in the genitourinary mucosa after immunization with a mucosal vaccine has been developed. In this method, secretory IgA antibody is measured by a highly sensitive enzyme immunoassay (immune-complex transfer enzyme immunoassay) using urine as a specimen. The urinary IgA antibody response could be detected by(More)
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