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Two large-scale yeast two-hybrid screens were undertaken to identify protein-protein interactions between full-length open reading frames predicted from the Saccharomyces cerevisiae genome sequence. In one approach, we constructed a protein array of about 6,000 yeast transformants, with each transformant expressing one of the open reading frames as a fusion(More)
OBJECTIVE To investigate the safety, pharmacokinetics (PK), binding activity and immunogenicity of CR002, a human monoclonal antibody (mAb) directed against platelet-derived growth factor-D (PDGF-D), administered as a single intravenous (i.v.) infusion over a range of doses. SUBJECTS 40 healthy male subjects received increasing doses of CR002 at 0.3, 1,(More)
Apical dominance in Xanthium strumarium was influenced by the quality of illumination received at the end of the photoperiod. The involvement of the red/far-red regions of the spectrum was apparent. The persistence of the effects was partially dependent on the age of the individual buds concerned. Plants receiving 30 minutes of illumination from tungsten(More)
X-ray microanatysis has revealed that the sodium content of the stomatal guard cells of Aster tripolium remains much lower than that of other leaf cells when the plants are grown at high salinity. Large amounts of sodium did, in contrast, accumulate in epidermal and subsidiary cells, and particularly in the mesophyll tissue, suggesting that a mechanism(More)
Regulation of the concentration of Ca2+ in the apoplast of plants is essential in order to allow Ca2+-dependent processes, such as Ca2+-mediated signal transduction in stomatal guard cells, to function correctly. This is particularly important for plants growing with high levels of Ca2+ in the rhizosphere. Recently, we have shown that in two calcicoles,(More)
Isolated epidermal strips of Commelina communis L. showed progressively smaller stomatal openings when incubated in abscisic acid solutions ranging in concentration from 10-8 to 10-4 M. The effects were reproducible and did not appear to be affected by the presence of auxin, gibberellic acid or kinetin. This specificity suggests that this method may prove(More)
Abscisic acid (ABA) at a concentration of 100 μm reduced the mean stomatal aperture on isolated epidermis of Commelina communis from 9.5 to 3.1 μm. This closure resulted from a fall in osmotic pressure of the guard cells from 14.1 to 9.8 bars; the osmotic pressure of the subsidiary cells did not change significantly. Histochemical tests showed that the(More)
Effects of fusicoccin alone and together with abscisic acid were observed on the stomatal complex of Commelina communis. The experimental material consisted of isolated epidermal strips incubated in a medium containing the ions required for stomatal opening. Fusicoccin stimulated opening and this was accompanied by potassium entry into the guard cells, and(More)
Sorghum plants that had been subjected to different degrees of water stress were examined for the occurrence of endogenous compounds capable of inducing stomatal closure, i.e. “antitranspirants”. Acidic extracts contained increased amounts of abscisic acid (ABA) as the amount of stress increased, but another highly active compound easily distinguished from(More)