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The nucleotide sequence of the promoter region of the gene for cholesterol oxidase (choA) from Streptomyces sp. strain SA-COO was determined. We found an open reading frame (choP) that is located between a potential promoter sequence and the structural gene for the ChoA protein. Deletion analysis showed that the promoter region for choP is essential for(More)
New antibiotics, enaminomycins A, B and C, were found in the culture broth of streptomycete strain No. 13120, which was identified as Streptomyces baarnensis and designated as S. baarnensis No. 13120. Fermentation of enaminomycins were performed by conventional submerged culture in a 30-liter jar fermentor. Isolation of the antibiotics was performed by(More)
The thiostrepton-resistance gene is expressed in Streptomyces jumonjinensis [16]-8 SANK 61185 carrying the plasmid pIJ702 but not the tyrosinase gene. We have isolated DNA fragments from various streptomycetes that restore expression of the tyrosinase gene on pIJ702 in this organism. The nucleotide sequences of two of these DNA fragments show that they(More)
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