T M Poliakova

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The authors present the results of study of chemical action on the specific properties of staphylococcus allergen showing that deamination, iodation, and formalinization was accompanied by reduction of the allergenic capacity of the preparation; as to acetylation -- it produced an insignificant reduction of the allergen activity.
Various forms of interaction between diphtheria exotoxin and the continuous L and HeLa cell lines were revealed, depending on its doses: toxic, subtoxic and small (following the subtoxic dose). Cells of the same origin, treated with these doses, develop similar changes in some of their properties, differing only in their "survival" time, the period of(More)
The authors suggest an optimal scheme of guinea pig sensitization for the assessment of specific activity of staphylococcal allergens. On milliard cells of killed staphylococcal culture are injected to the animals into the paws of the limbs; two and a half weeks later an intradermal test with an allergen in a dose of 10 microgram by protein was made.(More)
Allergens obtained from seven strains of staphylococcus cultures (with and without any toxigenic and hemolytic properties) were subjected to fractionation on Sephadexes. It was shown that allergens from strains 209, 3a, 977 contained one protein-polysaccharide, component with molecular weight of 600 000--900 000. Allergens from strains Wood-46, 150a, r-7,(More)
Various methods for obtaining staphylococcus allergens were approbated. Biological activity of the preparation proved to depend on the method of obtaining it determining the chemical compostition of the preparation, and on the properties of the strain used. A method of alkaline extraction and a strain with marked pathogenicity signs (strain 209) can be(More)
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