We propose a method to detect and track multiple moving biological spot-like particles showing different kinds of dynamics in image sequences acquired through multidimensional fluorescence microscopy. It enables the extraction and analysis of information such as number, position, speed, movement, and diffusion phases of, e.g., endosomal particles. The… (More)
In this Review, we describe the synthesis of high-quality colloidal nanoparticles in organic solvents, the mechanisms by which they can be transferred into aqueous solution, and some of their applications in biology. In particular, we will place emphasis on the creation of multifunctional nanoparticles or nanoparticle assemblies.
Acknowledgements First of all, I would like to thank my excellent advisor, Erik Winfree, who is full of great ideas. His challenge of building a general biochemical circuit turned out to be quite difficult. However, his enthusiasm and insight along with incessant support saved the day. One of my fond memories is building a cheap fluorometer machine with… (More)
The process of miniaturization brings benefits in many areas of every day life. It describes the process of down-scaling mechanical, optical and electronic devices while maintaining their function. This continuous process has brought up the field of nanotechnology. It allows computers to run faster and faster while reducing their size and energy… (More)