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A non-collagenous quantitatively prominent protein was purified from guanidine hydrochloride extracts of bovine tracheal cartilage. Purification was achieved by cesium chloride density gradient centrifugation and chromatography on DEAE-cellulose at pH 7.0 followed by CM-cellulose at pH 5.0. The protein has a marked tendency to form aggregates in denaturing(More)
This paper addresses the selection of controlled variables, that is, “what should we control”. The concept of self-optimizing control provides a systematic tool for this, and we show how it can be applied to the Tennessee Eastman process, which has a very large number of candidate variables. In this paper, we present a systematic procedure for reducing the(More)
We consider control structure selection, with emphasis on “what to control”, for a simple plant with a liquid-phase reactor, a distillation column, and recycle of unreacted reactants. Plants of this kind have been studied extensively in the plantwide control literature. Our starting point is a clear definition of the operational objectives, constraints, and(More)
Two proteins of Mr = 58,000 and 59,000, respectively, were purified from 4 M guanidinium chloride extracts of articular cartilage by dissociative CsCl-density gradient centrifugation followed by gel chromatography on Sephadex G-200 and ion exchange chromatography on DEAE-cellulose. The two proteins differ in ionic properties and only the one with Mr =(More)
Interactions between bovine chondrocytes and cartilage extracellular matrix proteins and proteoglycans were demonstrated by cell attachment to plastic surfaces coated with the macromolecules. Chondrocytes, which had been maintained in suspension culture, attached to fibronectin, bone sialoprotein, collagen II, and two novel 36- and 58-kDa proteins isolated(More)
Cultured bovine articular cartilage full thickness explants were mechanically loaded, both statically and cyclically, at high frequency (2s of load with 2s intervals of no load) and low frequency (60s of load with 60s intervals of no load), at 1 MPa. Metabolic effects of the load were studied by radiolabeling and compared with non-loaded cartilage explants.(More)
Time dependency in the mechanical properties of viscoelastic materials means that a variety of tests is often required to fully characterize their properties. Computer control is the best way of controlling loads and displacements and the rate at which they are applied, as well as recording and analysing the data produced. This paper describes apparatus for(More)
An early step in the biosynthesis of dermatan sulfate is polymerization to chondroitin, which then is modified by the D-glucuronyl C5-epimerase and mainly 4-O-sulfotransferase. The final structure of the dermatan sulfate side chains varies and our aim was to identify, which of the two enzymes that are crucial to generate dermatan sulfate copolymeric(More)
Most (if not all) available control theories assume that a control structure is given at the outset. They therefore fail to answer some basic questions that a control engineer regularly meets in practice (Foss 1973): “Which variables should be controlled, which variables should be measured, which inputs should be manipulated, and which links should be made(More)