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Microbodies, designated as peroxisomes because of their enzyme complement, have been isolated from spinach leaves. After grinding leaves in 0.5 M sucrose, the peroxisomes were removed with the broken chloroplast fraction by differential centrifugation. During sucrose density gradient centrifugation, the peroxisomes banded in about 1.9 M sucrose and were(More)
Aspergillus niger JTS 191 was selected from many microorganisms tested as capable of converting ionones to other compounds having aromas. The individual transformation products from beta-ionone were isolated and identified by comparison with synthetically derived compounds. The major products were (R)-4-hydroxy-beta-ionone and (S)-2-hydroxy-beta-ionone.(More)
gamma-Methylaminobutyraldehyde (N-methylpyrroline) labeled with (14)C was isolated from tobacco roots which had metabolized ornithine-2-(14)C. It was labeled most strongly 4 hours after adding ornithine-2-(14)C to the root, also labeled by putrescine-1,4-(14)C and methionine-(14)CH(3), and observed in the root but not in the aerial portions of tobacco(More)
Chloroplasts, mitochondria, and peroxisomes from leaves were separated by isopycnic sucrose density gradient centrifugation. The peroxisomes converted glycolate-(14)C or glyoxylate-(14)C to glycine, and contained a glutamate: glyoxylate aminotransferase as indicated by an investigation of substrate specificity. The pH optimum for the aminotransferase was(More)
Leaves of 10 plant species, 7 with photorespiration (spinach, sunflower, tobacco, pea, wheat, bean, and Swiss chard) and 3 without photorespiration (corn, sugarcane, and pigweed), were surveyed for peroxisomes. The distribution pattern for glycolate oxidase, glyoxylate reductase, catalase, and part of the malate dehydrogenase indicated that these enzymes(More)
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