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  • T J Dietz
  • The Journal of experimental biology
  • 1994
The characteristic heat shock or stress response is induced upon exposure of an organism (cell) to a number of environmental stressors, e.g. heat, anoxia and heavy metals (reviewed in Morimoto et al. 1990; Ellis and van der Vies, 1991). The heat shock or stress proteins are the most broadly distributed class of proteins known and amongst the most highly(More)
A protein fraction that requires the TATA sequence to bind to the hsp70 promoter has been partially purified from nuclear extracts of Drosophila embryos. This TATA factor produces a large DNase I footprint that extends from -44 to +35 on the promoter. A mutation that changes TATA to TATG interferes both with the binding of this complex and with the(More)
We monitored protein-DNA interactions that occur on the hsp26, hsp70, histone H3, and histone H4 promoters in nuclear extracts derived from frozen Drosophila melanogaster embryos. All four of these promoters were found to be transcribed in vitro at comparable levels by extracts from both heat-shocked and non-heat-shocked embryos. Factors were detected in(More)
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