T. I. Kuz'mina

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The comparative investigation of the individual and joint impact of prolactin (PRL, 50 ng/ml) and theophylline (TP), a nonselective inhibitor of phosphodiesterases, on nuclear maturation of bovine oocytes and the expansion of cumulus cells enclosing the oocytes was carried out using a model of in vitro culturing. It has been shown that TP (5 mM) exerts a(More)
Maturation of bovine oocyte-cumulus complexes (OCC) in media derived following granulosa cell culturing with prolactin (PRL, 50 ng/ml) and somatotropin (ST, 10 ng/ml) was studied. A medium conditioned by granulosa cells in the presence of PRL or ST exerted a stimulating effect on the proliferative activity of cumulus cells. ST introduction into the(More)
Immunogenicity for laboratory animals (rabbits and mice) of the whole hepatitis C virus envelope proteins and their conserved as well as hypervariable HVR1 sites has been investigated. Rabbit immune responses to HCV envelope proteins (both single E2 and E1E2 heterodimer) were shown to be much more efficient than murine immune responses. Upon the(More)
The influence of ryanodine and inositol triphosphate receptors inhibitors on Ca2+ exit from intracellular stores of porcine oocytes stimulated by prolactin and GTP was investigated using fluorescent dye chlortetracycline. Porcine oocytes were isolated from ovaries with yellow body. Ca2+ exit from intracellular stores of porcine oocytes activated by(More)
We studied the influence of bovine prolactin on the maturation of cumulus-enclosed bovine oocytes in different culture systems as well as on their capacity for subsequent development after in vitrofertilization. The prolactin effect on chromosome transformations in oocytes depended on the hormone concentration in the medium with fetal calf serum. Prolactin(More)
Bovine prolactin (BPRL) binding to cow granulosa cells depending on the antral follicular diameter has been studied. The data obtained point to the existence of one type of low affinity PRL-binding sites on granulosa cells. No reliable differences in the parameters of BPRL interaction with lactogenic receptors in the dynamics of folliculogenesis have been(More)
On the basis of inhibition analysis using inhibitors of protein kinase A and polymerization of microfilament under the influence oftheophylline, GDP and GTP have studied the release of Ca2+ from intracellular stores (ryanodin- and inositol-1 ,4,5-trisphosphate) of native and devitrified porcine oocytes. It is found that theophylline, as well as GDP(More)
The influence of bovine prolactin (BPL) on DNA synthesis in cultured cow granulosa cells in various media has been studied. BPL exerted a mitogenic effect on granulosa cells of follicles 3-5 mm in diameter, with the effect depending on hormone concentration in the cultural medium. In the medium with bovine growth hormone the maximum effective concentration(More)
Effect of estradiol on Ca2+ exit from intracellular stores in swine granulose cells activated by theophylline and prolactin is investigated with using fluorescent Ca2+ sensitive probe chlortetracycline. It was shown that prolactin and theophylline stimulated Ca2+ exit from intracellular stores. In absence of estradiol if we treatment of oocytes by(More)