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Mixed cortical and hypothalamic synaptosomes and striatal synaptosomes were allowed to accumulate 3H-norepinephrine and 3H-dopamine, respectively, for 5 min in the absence and presence of phencyclidine. The results indicate that phencyclidine probably decreased the accumulation of 3H-norepinephrine into mixed cortical and hypothalamic synaptosomes by only(More)
The dicarbamate felbamate has been shown to be capable of competing for the binding of 5,7-[3H]dichlorokynurenic acid ([3H]DCKA) to strychnine-insensitive glycine receptors in sections of human postmortem brain. The IC50 for this interaction was 305.8 microM and the inhibition was complete at 1 mM. Autoradiographic localization of [3H]DCKA binding revealed(More)
Phencyclidine was examined for its ability to release [3H]dopamine ([3H]DA) from prelabelled chopped rat striatal tissue. A dynamic perfusion system was used in order to minimize the effects of drugs on uptake mechanisms. Cocaine and S-(+)-amphetamine were used to distinguish uptake inhibition from a neurotransmitter releasing action. Phencyclidine,(More)
1. Study was performed to compare the role of prostaglandins in regulating gastric contractile activity in an amphibian model, Bufo marinus, with mammalian models. 2. The prostaglandin synthesis inhibitor, indomethacin, had little effect on spontaneous mechanical activity, but increased the force and frequency of contractions stimulated by acetylcholine. 3.(More)
[3H]5,7 Dichlorokynurenic acid ([3H]DCKA) was used to define conditions for obtaining selective binding to strychnine-insensitive glycine receptors. The parameters were established in sections of human brain prior to localizing the receptors sites by autoradiography. The binding of [3H]DCKA was of high affinity (Kd = 14.5 nM), readily reversible (K-1 =(More)
Intracellular microelectrode studies were conducted to examine the actions of the optical isomers levorphanol and dextrorphan on the isolated frog sartorius muscle preparation. Both isomers, in bath concentrations which had minimal effect on action potential amplitude and gNa (3 X 10(-5) M), caused a large depression of gK. High bath concentrations (3 X(More)
The positive therapeutic value of cuddling (petting, gentle stroking, fondling) has been appreciated by clinicians for many years; in fact, the practice of cuddling has its roots in lay therapy of antiquity. To our knowledge, no objective evaluation of the physiological and biochemical changes evoked by cuddling has yet been established. It was, therefore,(More)