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Expression plasmids carrying the coding sequence of mature human interleukin 1 beta (IL 1 beta) linked either to a Met start codon, or fused to different efficient Escherichia coli secretion signal sequences, have been constructed. In the latter case, we used signal peptides derived either from an outer membrane protein (OmpA) or from a periplasmic protein(More)
The optimization of fully automated sequencing reactions and analysis is a prerequisite for large scale sequencing programs. We describe here a very simple and reliable protocol which also solves all the evaporation problems encountered for sequencing reactions in microtiter plate. We used the property of the thermostable DNA polymerase from Thermophillus(More)
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