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An in vitro micronucleus assay was developed for primary cultures of rat hepatocytes and utilized to determine the oxygen enhancement ratio (OER). Freshly isolated Fischer 344 female rat hepatocytes were irradiated in suspension either in air or in anoxia, cultured for 60 h to allow for the maximum expression of micronuclei, fixed with methanol-glacial(More)
The radiosensitivity of human hepatocytes was determined and compared to that of rat hepatocytes. This interspecies comparison was performed by using the alkaline elution technique to measure DNA single-strand breaks and their repair in irradiated primary cultures of hepatocytes. Human hepatocytes obtained from discarded surgical material and Fischer 344(More)
To investigate the mechanism(s) of hepatocyte radioresistance (D0 2.7 Gy), the radiosensitivities of respiring (37 degrees C) and nonrespiring (0 degrees C) hepatocytes were determined as a function of oxygen concentration. Fischer 344 female rat hepatocytes were isolated by liver perfusion, equilibrated in Leibowitz-15 media with different oxygen tensions,(More)
When female Fischer 344 rats were fed a commercial diet (Purina Rodent Laboratory Chow 5010) the background sister chromatid exchange (SCE) level in hepatocytes significantly increased with time, whereas feeding a purified diet (TEKLAD TD 81050) led to no significant changes in the SCE frequency. These results are consistent with the hypothesis that the(More)
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