T. A. Voeikova

Learn More
The conjugal transfer of autonomous and integrative plasmids from the donor strain Escherichia coli S17-1 to strains of genera Actinomadura, Arthrobacter, Kitasatoa, Micromonospora, Nocardia, Rhodococcus, Saccharopolyspora, and to 16 strains of the genus Streptomyces was demonstrated. The status of plasmids in recipient strains and the stability of their(More)
New polyene macrolide S44HP was purified from the culture of recombinant Streptomyces noursei strain with engineered nystatin polyketide synthase. S44HP, nystatin (NYS), and amphotericin B (Amph-B) were tested against 19 clinical fungal isolates in agar diffusion assay, which demonstrated clear differences in antifungal activities of these antibiotics.(More)
Regulation of phenotypic variability of Bacillus licheniformis mediated by autoinducers of anabiosis, d1-factors was investigated. These factors are represented by alkylhydroxybenzenes. Colonial morphological variants of B. licheniformis were obtained and described (of R,S,M-types) in the first passage of both vegetative proliferative and resting cells.(More)
A system for introduction of plasmids into industrial producers of antibiotics chlortetracycline and bialaphos using intergeneric conjugation of Escherichia coli and Streptomyces was developed. Low level stability of inheritance of autonomously replicating DNA in recipient strains was shown. Site-specific integration of the conjugative-integrative vector(More)
The mutants of Shewanella oneidensis MR-1 resistant to fosfomycin, a toxic analogue of phosphoenolpyruvate, were obtained. The mutants exhibited increased reducing activity and higher rates of lactate utilization. A correlation was shown between the rates of metabolism of oxidized substrates and the rate of reduction of methylene blue, a mediator of(More)
Studies of regulation of antibiotic synthesis involved both basic and applied aspects of biology of antibiotic producers. This paper is a survey of published experimental data and key hypotheses for the regulation of antibiotic synthesis in bacteria of the genus Streptomyces. Summarized data on transcriptional regulatory systems of gene clusters responsible(More)
An expression plasmid was constructed in order to carry out heterologous expression of the gene of the NAD+-dependent formate dehydrogenase (FDH) from methylotrophic bacterium Moraxella sp. in the cells of Shewanella oneidensis MR-1 under aerobic and anaerobic conditions. In both modes of cell cultivation, recombinant FDH activity was revealed in the cell(More)
Analysis of phagolysates in production of fodder grisin provided determination of 3 serologically and morphologically different actinophages, 2 of which, i. e. Pg 81 and Pg 100 affected the sensitive culture as virulent phages and 1, i. e. Pg 2 affected it as a moderate phage. Investigation of the fermentation broth of strain 15 of Act. griseus did not(More)
Purpose of the work was designing and prototyping of microbial fuel cells (MFC) and comparative evaluation of the electrogenic activity of wastewater autochthonous microorganisms as well as bacterial monocultures. Objects were model electrogenic strain Shewanella oneidensis MR-1, and an Ochrobactrum sp. strain isolated from the active anode biofilm of MFC(More)