T. A. Stelmakh

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Sucrose gradient velocity centrifugation, polyacrylamide gel electrophoresis and RNA-RNA hybridization were used to characterize Lassa and Machupo virion RNAs as well as virus-specific RNAs from cells infected with Pichinde and Machupo viruses. Five RNA species: 30–31S, 28S, 22–24S, 18S and 4–6S have been detected in Lassa, Machupo, and Pichinde virion(More)
RNase resistance analyses of native, heat-denatured and self-annealed L and S species of Machupo virion RNA revealed the presence of complementary sequences. Self-annealing was concentration-dependent. The data indicate, that complementary sequences were present in separate RNA strands.
Molecular hybridization was used for the demonstration of virus-specific RNA synthesis in the latent phase of influenza virus replication in the sensitive MDCK cell cultures and for its investigation in the persistent system represented by nonpermissive AO cells and influenza A/Victoria 35/72 (H3N2).
RNA from Machupo virus infected cells was centrifuged in a linear sucrose gradient and RNAs from gradient fractions were tested separately for template activity in a cell-free protein synthesizing system from rabbit reticulocytes. Fraction 15–16 S programmed the synthesis of protein that migrated in SDS-polyacrylamide gel like the nucleocapsid protein of a(More)
Virus-specific RNA synthesis was studied in amniotic AO cells nonpermissive for influenza virus. Syntheses of complementary RNA were found to be greatly reduced in AO cells, as compared with the sensitive MDCK cells. Only primary transcription takes place in AO cells, resulting in the production of poly-A RNAs serving as a template for the synthesis of(More)
Single-stranded RNAs from Lassa virus-infected cells were fractionated by affinity chromatography on an oligo(dT)-cellulose column as well as by sucrose gradient centrifugation. Fractionated RNAs were translated in rabbit reticulocyte lysates, and translation products were precipitated with monoclonal antibodies to the nucleocapsid protein of Lassa virus.(More)
Cell-associated proteins of rodent (CG-1820 strain) and human (K-27 and 360 strains) hantaviruses isolated in the European endemic areas of the U.S.S.R. are antigenically similar as revealed by immunoprecipitation and immunoblotting assays. Nucleocapsid-associated RNAs of representative hantaviruses of four antigenic groups [Sugiyama et al. (1987) J Gen(More)
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