Sylvain Vignaud

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Quantitative reverse transcription-polymerase chain reaction (RT-PCR) used to detect minor changes in specific mRNA concentrations may be associated with poor reproducibility. Stringent quality control is therefore essential at each step of the protocol, including the PCR procedure. We performed inter-laboratory quality control of quantitative PCR between(More)
The cell type heterogeneity within clinical cancer tissue samples may affect the accuracy of gene expression analysis. In order to validate our laser microdissection (LMD) method using the Leica AS LMD system (LEICA Microsystems), we compared the mRNA levels of three major genes involved in breast cancer (ERalpha, PR, HER2), measured by means of real-time(More)
This paper address three different strategies (F-JPEG, F-DSC and F-YUV) for foveated image compression plus methods for the transmission of foveated image data. In place of simple subsampling for foveation, the proposed foveated compression strategies use varying compression ratios--a low compression ratio centrally, and progressively increasing compression(More)
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