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Inositol 1,4,5-trisphosphate (Ins(1,4,5)P3) induces Ca2+ oscillations and waves in Xenopus laevis oocytes. Microsomes from oocytes exhibit high-affinity binding for Ins(1,4,5)P3, and demonstrate Ins(1,4,5)P3-induced Ca2+ release. The Ins(1,4,5)P3 receptor (InsP3R) was purified from oocyte microsomes as a large tetrameric complex and shown to have a monomer(More)
The receptors for the second messenger inositol 1,4,5-trisphosphate [Ins(1,4,5)P3] form a family of closely related proteins that play an important role in regulating the free intracellular Ca2+ concentration. To test the hypothesis that changing the expression level of Ins(1,4,5)P3 receptors could alter the Ins(1,4,5)P3-mediated Ca2+ signal, we(More)
Stimulation of many nonexcitable cells by Ca2(+)-mobilizing receptor agonists causes oscillating elevations of the intracellular free Ca2+ concentration ((Ca2+]i), rather than a continuous increase. It has been proposed that the frequency at which [Ca2+]i oscillates determines the biological response. Because the occurrence of [Ca2+] oscillations is(More)
In object-oriented (OO) software engineering, objects, attributes and processes are often specified in natural language descriptions. Following a precise methodology, we here look at the analysis phase of OO software engineering and show that robust computational linguistic tools initially developed for knowledge extraction from text can provide useful(More)
Sentence syntax is the basis for organizing semantic relations in TANKA, a project that aims to acquire knowledge from technical text. Other hallmarks include an absence of precoded domain-specific knowledge; significant use of public-domain generic linguistic information sources; involvement of the user as a judge and source of expertise; and learning from(More)
Case systems abound in natural language processing. Almost any attempt to recognize and uniformly represent relationships within a clause – a unit at the centre of any linguistic system that goes beyond word level statistics – must be based on semantic roles drawn from a small, closed set. The set of roles describing relationships between a verb and its(More)
Stimuli which act through the second messenger inositol 1,4,5-trisphosphate (InsP3) often increase free intracellular Ca2+ concentration ([Ca2+]i) in a localized subcellular area. Actively propagated Ca2+ waves then extend this focal Ca2+ signal to other parts of the cell. To understand how cells may control the spatial distribution of Ca2+, we investigated(More)
Despite a large body of multidisciplinary research on helpful and user-oriented interface design, help facilities found in most commercial software are so ill-conceived that they are often 'unhelpful'. From a wide spectrum of disciplines and software tools, we present an extensive review of related work, identifying their limitations as well as their most(More)