Sylva Zelenková

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As sessile organisms, plants are unable to escape from the many abiotic and biotic factors that cause a departure from optimal conditions of growth and development. Low temperature represents one of the most harmful abiotic stresses affecting temperate plants. These species have adapted to seasonal variations in temperature by adjusting their metabolism(More)
Changes in cell viability, proliferation, cell and nuclear morphology including nuclear and DNA fragmentation induced by 0.05 and 1 mM CdSO4 (Cd2+) in tobacco BY-2 cell line (Nicotiana tabacum L.) were studied in the course of 7 days. Simultaneously changes in endogenous contents of both free and conjugated forms of polyamines (PAs) were investigated for 3(More)
Aluminum (Al) is a major factor that limits plant growth in acid soils. It causes a cessation of root growth and changes in root morphology suggesting a role of the root cytoskeleton as a target of Al-toxicity. Here we report a rapid effect of Al on the microtubular cytoskeleton of the suspension tobacco cell lines BY-2 and VBI-0. Viability studies showed(More)
Concurrently with cold-induced disintegration of microtubular structures in the cytoplasm, gradual tubulin accumulation was observed in a progressively growing proportion of interphase nuclei in tobacco BY-2 cells. This intranuclear tubulin disappeared upon rewarming. Simultaneously, new microtubules rapidly emerged from the nuclear periphery and(More)
Winter barley is an economically important cereal crop grown in higher latitudes and altitudes where low temperatures represent an important environmental constraint limiting crop productivity. In this study changes in proteome of leaves and crowns in a frost tolerant winter barley cv. Luxor in relation to short and long term periods of cold followed by a(More)
The accumulation of cold-induced dehydrin and proline was related to the frost tolerance (FT) in several Brassica species or cultivars. A dehydrin of molecular mass 47 kDa was detected in the leaves of an Ethiopian mustard (B. carinata) and a pair of dehydrins of similar molecular mass in the three (two winter, one spring) oilseed rape (B. napus) cultivars,(More)
Distant hybridization of several species of the genusAllium was performed. It was found that disharmony of the time of flowering of the parents may be overcome by storage of pollen ofA. fistulosum at 40 % humidity and 0 °C. Protein patterns of parents and F1 hybrids were compared using immunoelectrophoresis, esterase isoenzymes were evaluated by(More)
Molecular variability was determined between, 17Brassica napus cultivars, 8 new selections 4 F1 hybrids, 5 cultivars ofB. oleracea, B. campestris andB. peruvianum with 4 rDNA probes fromLycopersicon esculentum, 3 probes from a PstI library ofB. oleracea, 3 probes from EcoRI libraries ofB. napus andB. oleracea and the acetolactatsynthase gene fromNicotiana(More)
Karyotypes ofAllium fistulosum, A. altaicum andA. cepa cv. Karmen are described and protein patterns of their seeds determined by immunoelectrophoresis are compared. Esterase isoenzymes were evaluated by polyacrylamide gel electrophoresis and isoelectrofocusing. Karyological and chemotaxonomical analysis confirmed thatA. fistulosum andA. altaicum are(More)
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