Svetlana M. Kochubey

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Absorption maximum positions of three LW Chl forms in pea chloroplasts were estimated using 77 K excitation spectra of fluorescence detected in their maxima (720, 732 and 746 nm). The 705, 714 and 723 nm components were revealed in the second derivative plots of the excitation spectra. The same maxima were found in normalized excitation spectra obtained(More)
Reflectance spectra of leaves of winter wheat, maize, sugar beet and wild vine have been measured. Chlorophyll variations induce changes in the first derivatives of the reflectance spectra. A correlation of various derivative indices such as D(725)/D(702), D(lw)/D(sw), D(lw)/D(702), D(725)/D(sw,)D(715)/D(702), D(m)/D(sw), D(m)/D(702) and D(715)/D(sw) with(More)
Changes in low-temperature fluorescence spectra of pea chloroplasts induced by the short-term heating were studied. Excitation spectra of the long-wavelength fluorescence were studied as well. Heating was carried out at 45°C for 5 min in the darkness or in the presence of white light sourced with intensities of 260 or 1400 μmol/m2 s. All variants of heating(More)
A lipophilic nitroxyl radical, 1-oxyl-2,2,6,6-tetramethylpiperidin-4-yl 1-adamantylacetate, has been applied to EPR spin probe study of chloroplasts and subchloroplast fragments of different types. The latter originate from grana and the grana core regions. The binding of the spin probe to the membranes was revealed by specific changes in a shape of the EPR(More)
Changes in antenna of photosystem II, induced by short-term heating, were studied using characteristics of a short-wavelength band in low-temperature fluorescence spectra (77 K) of pea chloroplasts. Heating for 5 min was carried out at 25 and 45°C in the darkness or in the presence of white light with intensity of 260 or 1,400 μmol/m2s. Most modes of(More)
The photosynthetic membrane composition and low temperature fluorescence spectra were analyzed for pea chloroplasts from control and clinostated plants. Clinorotation induces a decrease in the amount of the oligomeric form of the light-harvesting chlorophyll a/b complex (LHCII) and an increase of its monomeric form. Some changes in organization of(More)
Changes in lipid composition of Photosystem 1 (PS 1) particles isolated from thylakoids phosphorylated under reductive or anaerobic conditions have been studied. Under reductive conditions, there was an increase in monogalactosyldiacylglycerol containing highly saturated fatty acids and phosphatidylglycerol containing transhexadecenoic fatty acid. Under(More)
Heavy fragments were isolated from pea chloroplasts using digitonin treatment and differential centrifugation. The particles were characterized by a significantly lowered chlorophyll a/b ratio, contents of photosystem I (PS I) proteins and ATPase, as well as of amount of P700. The content of photosystem II (PS II) proteins decreased insignificantly, whereas(More)
The early fight-inducible proteins (ELIPs) are nuclear-encoded proteins localized in the chloroplasts and related to cab (light harvesting chlorophyll alb-binffmg) gene family. Light stress controls ELIP expression at different levels, at the level of transcription and the accumulation of the protein in the thylakoid membranes. The induction of ELIP(More)