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Flow cytometric analysis of DNA content was done on 133 primary squamous cell carcinomas of the larynx. Overall, 76 tumors (57.1%) were not diploid (aneuploid or tetraploid). The DNA index (DI) was calculated and grouped into three levels. Fifty-seven cases had a DI in a range of 1.85 to 2.15 (44 diploid and 13 tetraploid). The cases were grouped in(More)
Cytochemical methods using silver proteinate, silver methenamine and potassium ferrocyanide + OsO4 for ultrastructural detection of glycoproteins allow, in the posthypophysis and the magnocellular nuclei of the rat, differentiation of two types of fibres and neurons: one type containing negative granules with a homogeneous content of low electron density,(More)
Incubation in a culture medium with and without TSH of 16 day-old foetal thyroid glands induces hypertrophy of the Golgi apparatus which may be correlated with a considerable increase in the number of secretory vesicles. A stereological study performed during the first 6 hr of incubation showed that: vesicle secretion was biphasic; vesicle secretion was(More)
The hypothalamo-posthypophysial complex of the homozygous Brattleboro rat is characterized by a hyperactivity of its neurons. The neurosecretory fibers, especially in the neurohypophysis, show numerous signs of autophagy and tubular proliferation of the axoplasmic reticulum. These structural alterations, as well as the nematosomes or nucleolus-like bodies(More)
BACKGROUND DNA flow cytometry of hepatocellular carcinoma (HCC) cells has been investigated in many studies, but, to the best of our knowledge, there are no data on DNA analysis of cirrhotic parenchyma around the HCC. In this study, cell kinetics and ploidy of parenchymal cells around HCC were performed to ascertain if this would predict the possibility of(More)
In the medico-legal practice differential diagnosis between spontaneous and non-spontaneous abortion is important because causes of pregnancy wastage are often obscure and, moreover, spontaneous abortion is more common than accidental or voluntary. In all the cases in which the cause of abortion is not otherwise detectable and especially in cases of(More)
By flow cytometric analysis of nuclear DNA content it is possible to demonstrate the cellular clones that have a quantity of DNA higher or lower in comparison with that of diploid nuclei. The possibility of measuring DNA ploidy by flow cytometry provides a simple way to asses triploidy and tetraploidy in placental tissue. In this investigation, are reported(More)
When glucose is added to the culture medium, some cells of the undifferentiated HT-29 line derived from a human colonic adenocarcinoma develop spherical structures, demonstrated to be intracellular by the ruthenium red staining method, which are bordered with microvilli, contain osmiophilic substances and resemble intracellular lumina. When glucose is(More)