Suzanna Pokhil

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In preliminary ELISA studies where released-active forms (RAF) of antibodies (Abs) to interferon-gamma (IFNg) were added to the antigen-antibody system, a statistically significant difference in absorbance signals obtained in their presence in comparison to placebo was observed. A piezoelectric immunosensor assay was developed to support these data and(More)
The lipopolysaccharide of a new species of Enterobacteriaceae, Rahnella aquatilis 95U003, was isolated and investigated. The structural components of the lipopolysaccharide molecule, lipid A, core oligosaccharide, and O-specific polysaccharide, were isolated by mild acidic hydrolysis. In lipid A, 3-hydroxytetradecanoic (64.3%) and tetradecanoic (22.3%)(More)
The technique of making paper disks with Klebsiella bacteriocins is described, as is the method for improving the stability of bacteriocin preparations. The authors have developed a method for bacterial bacteriocin typing, facilitating the work and taking but 4-5 hrs. The results of klebocin typing of Klebsiella-237 strains with the use of the developed(More)
The paper presents main biological characteristics of six new species of enterobacteria, which may cause a number of infectious processes in man: Moellerella wisconsensis, Koserella trabulsii. Leclercia adecarboxylata. Escherichia fergusonii. Rahnella aquatilis, Enterobacter asburiae. The characteristics presented allow the bacteriologists of the laboratory(More)
Hemolytic activities of Klebsiella-237 strains of various origins were measured in solid nutrient media by three methods making use of six different agar bases for the preparation of blood nutrient media. The authors analyze the agar bases, discuss the advantages and shortcomings of all the methods used with consideration for the purpose of the(More)
LPS have been isolated from 6 strains of Rahnella aquatilis, their separate structure components have been obtained: lipid A, O-specific polysaccharide shain (OPS) and core oligosaccharide. 3-Oxytetradecanoic (31.7%) and tetradecanoic (17.8%) acids are dominating fatty acids of the lipid A. The core oligosaccharide of most investigated strains includes(More)
Lipopolysaccharides (LPSs) of two strains Pragia fontium 97U116 and 27480 were isolated and characterized; they were close to those of other representatives of the family Enterobacteriaceae in fatty acid composition and contained, respectively, 3-hydroxytetradecanoic acid as the predominant component (45.8 and 45.1%), tetradecanoic (23.5 and 28.9%),(More)
The paper deals with the results of analysis of 219 strains of Klebsiella using 12 tests--fermentation of adonite, asparaginic acid, sodium citrate, dulcite, d-tartrate, glutamic acid, inosite, L-proline, sodium malonate; reactions with methyl red, Foges-Proscauer, with 5-ASA. A scheme for determining fermentovars of Klebsiella is suggested which includes(More)