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A catechol 1,2-dioxygenase (CD) was found, which was synthesized constitutively in the aniline-assimilating bacterium Rhodococcus sp. AN-22 grown on a medium without aniline, as well as on aniline medium. The bacterium synthesized CD in its cells grown on all the 21 non-aromatic substrates examined, including four natural media such as meat and yeast(More)
Peroxisome proliferators are known to modulate the activity of xenobiotic-metabolising enzymes, including glutathione S-transferase (GST) and cytochrome P-450 (CYP). In this study the effect of peroxisome proliferators silvex and di(2-ethylhexyl)phthalate (DEHP) on the formation of (+)-anti-benzo(a)pyrene -7,8-dihydrodiol-9,10-epoxide (BPDE)-DNA adducts(More)
trans-7,8-Dihydroxy-9,10-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene [(+)-anti-BPDE] is rapidly inactivated in aqueous solvents due to hydrolysis to tetraols. No significant effect on the rate of hydrolysis is observed in the presence of glutathione (GSH)-depleted cytosol. However, when the cytosolic fraction is replaced by a mixture of glutathione(More)
Glutathione S-transferase (GST) isoenzymes of rat liver cytosol were purified from a S-hexylglutathione Sepharose affinity column without using S-hexyl-glutathione for elution. The method involves an initial elution of GSTs 3-4 and 4-4 from the affinity matrix with 10 mM glutathione in 50 mM Tris-HCl (pH 7.0), followed by the elution of GSTs 1-1, 1-2, 2-2(More)
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