Susanna M. Lewis

Learn More
Estimates put the origin of V(D)J recombination at ‫ف‬ 450 million years ago (for reviews, see references 1, 2). It has been speculated that it all started with a chance occurrence, the integration of a mobile element into a gene encoding an Ig domain (3). The acquisition of the V(D)J recombination system represented a major advance in the biological arms(More)
DNA double-stranded breaks (DSBs) can be repaired by several mechanisms, including classical NHEJ (c-NHEJ) and a poorly defined, error-prone process termed alternative NHEJ (a-NHEJ). How cells choose between these alternatives to join physiologic DSBs remains unknown. Here, we show that deletion of RAG2's C-terminus allows a-NHEJ to repair RAG-mediated DSBs(More)
This report summarizes the proceedings of the one day BioSharing meeting held at the Intelligent Systems for Molecular Biology (ISMB) 2010 conference in Boston, MA, USA This inaugural BioSharing event was hosted by the Genomic Standards Consortium as part of its M3 & BioSharing special interest group (SIG) workshop. The BioSharing event included invited(More)
two-site interaction that results in inversion or deletion, as in CSSR, however, the two junctions that are pro-* Division of Immunology and Cancer Hospital for Sick Children Research Institute duced, (a " coding joint " and a " signal joint ") are not exactly reciprocal. There is usually a gain and/or loss † The Wellesley Hospital Research Institute ‡(More)
The nature of any long palindrome that might exist in the human genome is obscured by the instability of such sequences once cloned in Escherichia coli. We describe and validate a practical alternative to the analysis of naturally-occurring palindromes based upon cloning and propagation in Saccharomyces cerevisiae. With this approach we have investigated an(More)
Archives MASSACHIUSETTS INSTITIUW OF TECHNOLOGY FEB 9 1-3-LIBRARIES 2 To my parents Joe and Sarah and to Charles "Ned" Holt Acknowledgements I would like to express my sincere thanks to Graham Walker, my advisor, for his constant encouragement and support especially in the early stages of this work. I would like to thank him for the many discussions, ideas,(More)
  • 1