Susana Manzano

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Recent molecular and genomic studies carried out in a number of model dioecious plant species, including Asparagus officinalis, Carica papaya, Silene latifolia, Rumex acetosa and Marchantia polymorpha, have shed light on the molecular structure of both homomorphic and heteromorphic sex chromosomes, and also on the gene functions they have maintained since(More)
The study of the molecular structure of young heteromorphic sex chromosomes of plants has shed light on the evolutionary forces that control the differentiation of the X and Y during the earlier stages of their evolution. We have used the model plant Rumex acetosa, a dioecious species with multiple sex chromosomes, 2n = 12 + XX female and 2n = 12 + XY1Y2(More)
Sex chromosomes are an ideal system to study processes connected with suppressed recombination. We found evidence of microsatellite expansion, on the relatively young Y chromosome of the dioecious plant sorrel (Rumex acetosa, XY1Y2 system), but no such expansion on the more ancient Y chromosomes of liverwort (Marchantia polymorpha) and human. The most(More)
We have identified a kind of parthenocarpy in zucchini squash which is associated with an incomplete andromonoecy, i.e. a partial conversion of female into bisexual flowers. Given that andromonoecy in this and other cucurbit species is caused by a reduction of ethylene production in the female flower, the associated parthenocarpic development of the fruit(More)
Ethylene is an essential regulator of flower development in Cucurbita pepo, controlling the sexual expression, and the differentiation and maturation of floral organs. To study the action mechanism of ethylene during the male and female flower development, we have identified two CTR1 homologues from C. pepo, CpCTR1 and CpCTR2, and analysed their expressions(More)
To optimize the sanitation treatment of ready-to-eat (RTE) intermediate-moisture foods (IMF), the behavior of Listeria monocytogenes Scott A (CIP 103575), L. innocua (NTC 11288), Salmonella enterica serovar Typhimurium (CECT 443), and Escherichia coli O157:H7 (CECT 4972) following treatment with electron-beam irradiation has been studied. As food matrixes,(More)
A rapid method for the estimation of the microbiological quality of refrigerated raw milk was developed based on the aminopeptidase activity of Gram-negative psychrotrophic bacteria, the dominant microbial population in refrigerated foods. Two versions of the test for measuring the aminopeptidase activity are reported (direct and indirect). A good(More)
Parthenocarpy is becoming an essential trait for off-season greenhouse production of Zucchini squash. Given that winter conditions promote a reduction in the number of male flowers and in the activity of pollinators, the application of synthetic auxins is currently the most widespread method to induce fruit set. We have evaluated the parthenocarpic tendency(More)
This is a study on the efficacy of pulsed light (PL) technology for the inactivation of Salmonella enterica serovar Enteritidis on shell eggs. In preliminary studies on noble agar, a PL treatment of 0.7 J/cm(2) gave an inactivation of 6.7 log CFU/cm(2). Photoreactivation of Salmonella (0.5-0.7 log CFU/cm(2)) was observed. Different results were obtained in(More)
Sex determination in Rumex acetosa, a dioecious plant with a complex XY1 Y2 sex chromosome system (females are XX and males are XY1 Y2 ), is not controlled by an active Y chromosome but depends on the ratio between the number of X chromosomes and autosomes. To gain insight into the molecular mechanisms of sex determination, we generated a subtracted cDNA(More)