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Staphylococcus aureus is a bacterial pathogen causing approximately 80% of all cases of human osteomyelitis. This bacterium can adhere to and become internalized by osteoblasts and previous studies indicate that osteoblasts are active in the internalization process. In the current study, we examined the roles of microfilaments, microtubules and(More)
Multiple spliced transcripts of human papillomavirus type 1 were detected by electron microscopic analysis of R-loops formed with total RNA extracted from plantar warts and with poly(A)+ RNA isolated from cultured keratinocytes infected with human papillomavirus type 1. The 5' ends of the RNAs were mapped to sites in the E7 open reading frame (ORF), just(More)
Staphlylococcus aureus is the primary pathogen associated with osteomyelitis, an acute and recurrent bone disease. Internalization of S. aureus by cultured embryonic chick calvarial osteoblasts has been observed. The purpose of this study was to demonstrate that internalization of bacteria by embryonic chick calvarial and tibial osteoblasts occurs in vivo.(More)
Staphylococcus aureus invades osteoblasts and is the primary cause of osteomyelitis. This study examined the ability of S. aureus to induce apoptosis in a mouse osteoblast cell line. The presence of intracellular S. aureus was demonstrated by transmission electron microscopy. Light microscopy was utilized to examine morphological changes in the osteoblasts(More)
Cells of a strain of Lactobacillus lactis selected for ability to produce hydrogen peroxide were added to Trypticase soy broth (TSB) containing Escherichia coli O157:H7 to determine if L. lactis was antagonistic towards the E. coli during storage at 7 degrees C for 7 days. E. coli was enumerated on violet red bile agar. Three strains of E. coli O157:H7(More)
We have examined the growth of three epitheliotropic viruses in cultures of human epidermal keratinocytes: herpes simplex virus (HSV) type 1, adenovirus type 2 (Ad-2), and human papillomavirus (HPV) type 1. Differences were noted in the level of expression of each virus, and these differences may be related to a dependency or lack of dependency on(More)