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A generally applicable, rapid, and sensitive method for profiling and sequencing of glycoprotein-associated N-linked oligosaccharides from protein gels was developed. The method employed sodium(More)
Sialylated carbohydrates usually decompose by loss of sialic acid when ionized by matrix-assisted laser desorption/ionization (MALDI) as the result of the labile carboxylic proton. Stabilization has(More)
This paper describes a sensitive method for analysis of N-linked carbohydrates released enzymatically from within the gel following separation of glycoproteins (50-100 pmols) by sodium dodecyl(More)