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Divalent metal ions are essential for the folding and catalytic activities of many RNAs. A commonly employed biochemical technique to identify metal-binding sites in RNA is the rescue of Rp alpha-phosphorothioate (PS) interference by the addition of soft divalent metal ions. To access the ability of such experiments to accurately identify metal-ion(More)
Plant polygalacturonase-inhibiting proteins (PGIPs) belong to the leucine-rich repeat (LRR) family and are known to prevent pathogen invasion by inhibiting the plant cell wall degrading enzyme, polygalacturonase. Our study reveals that these multigene-encoded defence proteins found in flowering plants only exhibit identical domain architecture with 10(More)
Whole genome duplication leads to autopolyploidy and brings about an increase in cell size, concentration of secondary metabolites and enhanced cytosine methylation. The increased cell size offers a positive advantage to polyploids for cell-surface-related activities, but there is a differential response to change in body size across species and taxonomic(More)
Metal ions are essential for the folding and activity of large catalytic RNAs. While divalent metal ions have been directly implicated in RNA tertiary structure formation, the role of monovalent ions has been largely unexplored. Here we report the first specific monovalent metal ion binding site within a catalytic RNA. As seen crystallographically, a(More)
Many RNAs, including the ribosome, RNase P, and the group II intron, explicitly require monovalent cations for activity in vitro. Although the necessity of monovalent cations for RNA function has been known for more than a quarter of a century, the characterization of specific monovalent metal sites within large RNAs has been elusive. Here we describe a(More)
Environmental stress in plants impacts many biological processes, including male gametogenesis, and affects several cytological mechanisms that are strongly interrelated. To understand the likely impact of rising temperature on reproductive fitness in the climate change regime, a study of tapetal mitosis and its accompanying meiosis over seasons was made to(More)
  • S Basu
  • 1985
Evidence has been presented to prove that cathodoluminescence (CL) studies of chromosomes and spread, Giemsa stained chromatin may lead to early detection of structural changes, such as the superstructure of heterochromatin. Several compounds including the potent hallucinogenic drugs, LSD and cocaine, have been discovered to be cathodoluminescent. The main(More)
  • S Basu
  • 1985
Wet replication studies of potato mitochondria suggest that these mitochondria are basically elongated organelles of length 3-6 micron and width 0.5-0.9 micron. However due to unnatural extracellular and environmental conditions these mitochondria are subject to spontaneous segmentation or fission to yield organelles of smaller forms, and finally to round(More)