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We have recently shown the presence of alpha B-crystallin in non-ocular tissues of diverse embryological origins such as the heart, brain, spinal cord, kidney, retina, etc. Using an alpha B-crystallin-specific antiserum and immunofluorescence, immunoblotting, immunoprecipitation and peptide mapping with Staphylococcus aureus protease, we demonstrate(More)
The molecular cascade of stress response in higher eukaryotes commences in the cytoplasm with the trimerization of the heat shock factor 1 (HSF1), followed by its transport to the nucleus, where it binds to the heat shock element leading to the activation of transcription from the down-stream gene(s). This well-established paradigm has been mostly studied(More)
We have cultured and maintained human fetal lens epithelial cells for several months in primary, secondary, and tertiary culture(s). These cells show unabated synthesis of alpha B-crystallin (alpha B), a lens epithelial cell-specific marker, and progressive expression of beta Bp-crystallin (beta Bp), a major polypeptide of the differentiated lens fiber(More)
PURPOSE During the early phase of experimental autoimmune uveitis (EAU), before macrophages infiltrate the retina and uvea, photoreceptor mitochondrial oxidative stress, nitration of photoreceptor mitochondrial proteins, and release of cytochrome c have been observed. However, no apoptosis has been detected during this phase. In this study,(More)
The nucleotide sequence of a cloned cDNA derived from gamma-crystallin mRNA of calf lens was determined. The cloned cDNA contains the entire coding region 522 bp long, 30 nucleotides of the 5' noncoding region, and 67 residues in the 3' noncoding region followed by a poly(A) tail of 25 nucleotides. The deduced amino acid sequence directly demonstrates for(More)
Double-stranded cDNA synthesized from delta-crystallin mRNA isolated from lens fiber cells of 15-day-old embryonic chicken was cloned in Escherichia coli chi 1776 in the Pst I site of the plasmid pBR322 by using the oligo(dC) . oligo(dG) joining procedure. Twelve Amps Tetr transformants contained sequences complementary to purified delta-crystallin(More)
alpha-Crystallin, a tissue specific structural protein of the ocular lens, is known to be composed of two subunits, alpha A and alpha B. By using a specific antibody in an immunoblotting procedure we have found that one of the subunits, alpha B is present in a number of non-lenticular tissues including the retina, heart, skeletal muscle, skin, brain, spinal(More)
alpha-Crystallin, the predominant structural protein of the ocular lens, has been considered to be composed of two subunits, alpha A-crystallin and alpha B-crystallin. Of these two, alpha B-crystallin has been previously shown to be an extralenticular protein while alpha A-crystallin has been considered to be a lens-specific polypeptide. Using an antiserum(More)
An adult lens contains two easily discernible, morphologically distinct compartments, the epithelium and the fiber-cell mass. The fiber-cell mass provides the lens with its functional phenotype, transparency. Metabolically, in comparison to the fiber cells the epithelium is the more active compartment of the ocular lens. For the purposes of this review we(More)