Suphot Wattanaphansak

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Equine proliferative enteropathy (EPE) caused by Lawsonia intracellularis has recently been recognized as an emerging disease in foals. Whilst the clinical entity, diagnostic evaluation and treatment of affected foals have been well established and described, preventive measures for EPE have remained largely unaddressed. The objectives of this study were to(More)
BACKGROUND Equine proliferative enteropathy (EPE) is an emerging disease of weanling foals. OBJECTIVES Describe clinical, hematologic, biochemical, serologic, molecular, and ultrasonographic findings in foals experimentally infected with Lawsonia intracellularis. ANIMALS Eight foals. METHODS Recently weaned foals were assigned to either the challenge(More)
The objective of this study was to develop an indirect enzyme-linked immunosorbent assay (ELISA) using a sonicated pure culture of Lawsonia intracellularis as the antigen (So-ELISA). A total of 332 serum samples, consisting of 232 experimentally infected animals and 100 animals naturally infected with L. intracellularis, were used to assess the diagnostic(More)
An alternative method for the cultivation of Lawsonia intracellularis, an obligate intracellular bacterium and the causative agent of proliferative enteropathy, was developed using an Original Space Bag inflated with a mixture of gas containing 10% hydrogen, 10% carbon dioxide, and 80% nitrogen. The flexibility of this protocol allows the testing of various(More)
The objective of this study was to determine the in vitro minimum inhibitory concentration (MIC) of antimicrobials against 10 isolates of Lawsonia intracellularis, the etiological agent of proliferative enteropathy (PE). Antimicrobials tested included carbadox, chlortetracycline, lincomycin, tiamulin, tylosin and valnemulin. The MIC of each antimicrobial(More)
The objective of this study was to develop and test both a flow cytometry method (FCM) and a direct count method (DCM) that both use fluorescent stains to determine the viability of Lawsonia intracellularis (LI), an obligate intracellular bacterium and the cause of proliferative enteropathy (PE) in pigs and other animal species. Live LI were passaged in(More)
Suphot Wattanaphansak Supol Luengyosluechakul Alejandro Larriestra John Deen THE IMPACT OF CROSS-FOSTERING ON SWINE PRODUCTION Cross-fostering is a technique used in swine management systems to reduce preweaning mortality rates, increase growth rates and reduce weight variations in litters. However, extensive cross-fostering and moving piglets from one(More)
The objective of the present study was to develop a quantitative polymerase chain reaction (qPCR) assay using SYBR Green for quantification of Lawsonia intracellularis in cell culture and pig fecal samples. Specific primers were designed and tested using the aspartate ammonia-lyase (aspA) gene as a target. Serial 10-fold dilutions of cell culture samples(More)
Materials and methods: Two L intracellularis isolates were tested for susceptibility to several classes of disinfectants, including iodine, a biguanide, phenol, an oxidizing agent, a quaternary ammonium compound (QAC), and the combinations of QACs with aldehydes (formaldehyde and glutaraldehyde). All disinfectants were diluted with simulated hard water by(More)
The objective of this study was to determine the in vitro efficacy of Stalosan F, a mixed chemical and heavy metal disinfectant, against two strains of Lawsonia intracellularis using both a modified tissue culture and a direct count method. For testing as a powder, 1g, 0.5g, or 0.25g of Stalosan F was applied to bacterial solutions spread into sterile(More)