Supat Attathom

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A new tospovirus isolated from naturally infected tomato plants grown in Nakhon Pathom province (Thailand) was characterized. Infected plants showed symptoms consisting of necrotic spots, necrotic ringspots and stem necrosis. This virus was detected using general antibodies that could recognize watermelon silver mottle virus (WSMoV), capsicum chlorosis(More)
Three isolates of sugarcane mosaic virus (SCMV) from sugarcane and maize in Thailand, were selected and used as viral sources for coat protein (CP) genes cloning by immunocapture reverse transcription polymerase chain reaction (IC-RT-PCR). The CP gene sequences and their deduced amino acids were determined. The sequences of three cloned CP genes, UT6TH-sgc(More)
A virus isolated from chilli pepper plants in Kamphaengsaen, Nakorn Pathom, showing vein banding mottle symptoms was classified using sequence analysis and phylogeny of the coat protein gene and 3′ noncoding region (3′ NCR). This virus was found to be a typical potyvirus on the basis of particle morphology, biological properties and cytopathology. The 3′(More)
Bipartite geminiviruses infecting tomatoes in Thailand were detected by polymerase chain reaction (PCR) using CPA5/CPA2 primers. Products derived from PCR-amplified full-length DNA-A and DNA-B of TYLCV collected from Chiang Mai, Nong Khai, and Sakon Nakhon were cloned and sequenced. DNA-A from Chiang Mai was 2747 nts long; Nong Khai, 2744 nts; and Sakon(More)
Nucleotide sequences of the three distinct Tomato yellow leaf curl Thailand virus (TYLCTHV) strains (CM, NK, SK) were analyzed for recombination events. Recombination detection program analyses and a sequence alignment survey provided evidence of recombination between AC1 sequences of TYLCV, TYLCTHV-[MM], and TYLCTHV-[NK] as major parents and of ToLCLV,(More)
The DNA-binding activity and DNA-binding domain of Tomato yellow leaf curl Thailand virus coat protein were investigated. A full-length coat protein (CP) and two truncated derivatives lacking the amino (CPΔ1-62) and carboxyl (CPΔ126-257) termini were produced in Escherichia coli as fusion proteins to glutathione-S-transferase (GST). Southwestern analysis(More)
A phytoplasma was detected in annual blue grass (Poa annua L. Fienardo), exhibiting white leaf symptoms, that was grown in the fields near Caserta in southern Italy. Based on restriction fragment length polymorphism analysis of PCR-amplified 16S rDNA sequences, the phytoplasma associated with annual blue grass white leaf disease was identified as a new(More)
Tomato yellow leaf curl Thailand virus, TYLCTHV, is a begomovirus that causes severe losses of tomato crops in Thailand as well as several countries in Southeast and East Asia. The development of monoclonal antibodies (MAbs) and serological methods for detecting TYLCTHV is essential for epidemiological studies and screening for virus-resistant cultivars.(More)
The success of the Journal of General Plant Pathology largely depends on the quality of manuscripts submitted by authors, on the careful review by associate editors, and on the painstaking proofreading by editorial managers. We express special and sincere appreciation to Dr. Beth E. Hazen (U.S.A.) for her great efforts in correcting the English in the(More)
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