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Dual targeting of a single tRNA(Trp) requires two different tryptophanyl-tRNA synthetases in Trypanosoma brucei.
RNA interference analysis established that both Trp RS1 and TrpRS2 are essential for growth and required for cytosolic and mitochondrial tryptophanyl-tRNA formation, respectively, and the notion that, in an organism, all nuclear-encoded tRNAs assigned to a given amino acid are charged by a single aminoacyl-t RNA synthetase is not universally valid. Expand
Biosynthesis of phosphoserine in the Methanococcales.
All methanogenic archaea apparently share this pathway, providing sufficient phosphoserine for the tRNA-dependent cysteine biosynthetic pathway, and this enzyme catalyzed the transamination of aspartate, glutamate,osphoserine, alanine, and cysteate. Expand
Effect of selected Ser/Ala and Xaa/Pro mutations on the stability and catalytic properties of a cold adapted subtilisin-like serine proteinase.
A subtilisin-like serine proteinase from a psychrotrophic Vibrio species (VPR) shows distinct cold adapted traits regarding stability and catalytic properties, while sharing high sequence homologyExpand
Mutational analysis of Sep‐tRNA:Cys‐tRNA synthase reveals critical residues for tRNA‐dependent cysteine formation
This work mutated 19 conserved residues in Methanocaldococcus jannaschii SepCysS, and employed an in vivo system to determine the activity of the resulting variants, providing insights into the catalytic and substrate binding mechanisms of this poorly characterized enzyme. Expand
Rational design and directed evolution of a bacterial-type glutaminyl-tRNA synthetase precursor
An engineered enzyme variant (M110) with seven amino acid changes is able to rescue growth of the temperature-sensitive Escherichia coli glnS strain UT172 at its non-permissive temperature, implying that GluRS2 is a few steps away from evolving into a GlnRS and provides a paradigm for studying aminoacyl-tRNA synthetase evolution using directed engineering approaches. Expand
Dual Targeting of a tRNAAsp Requires Two Different Aspartyl-tRNA Synthetases in Trypanosoma brucei*
Mitochondrial Tb-AspRS2 defines a novel group of eukaryotic AspRSs with an expanded substrate specificity that are restricted to trypanosomatids and therefore may be exploited as a novel drug target. Expand
Mitochondrial tRNA import in the parasitic protozoon Trypanosoma brucei and its consequences on mitochondrial translation
The mitochondrial inner and outer membranes are composed of a variety of integral membrane proteins, assembled into the membranes post-translationally. The small TIMs are a group of ~10 kDa proteinsExpand
Iron Status of Icelandic Blood Donors and the Impact of Different Ferritin Assay Platforms on Donor Deferral Rates
The proportional bias observed between the two instruments/laboratories for ferritin assays underlines the importance of taking instrument-related performance into account when establishing ferritIn cut-offs for blood donor deferral. Expand