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Pronounced cohabitation of active immunoglobulin genes from three different chromosomes in transcription factories during maximal antibody synthesis.
To understand the relationships between nuclear organization and gene expression in a model system, we employed three-dimensional imaging and chromatin immunoprecipitation (ChIP)-chromosomeExpand
Vκ Gene Repertoire and Locus Contraction Are Specified by Critical DNase I Hypersensitive Sites within the Vκ-Jκ Intervening Region
It is demonstrated that a 650-bp sequence corresponding to DNase I hypersensitive sites HS1–2 within the mouse Igκ gene V-J intervening region binds CCCTC-binding factor and specifies locus contraction and long-range Vκ gene usage spanning 3.2 Mb in pre-B cells. Expand
Charged Amino Acid-rich Leucine Zipper-1 (Crlz-1) as a Target of Wnt Signaling Pathway Controls Pre-B Cell Proliferation by Affecting Runx/CBFβ-targeted VpreB and λ5 Genes*
The mechanistic view that the proliferative function of Crlz-1 is caused by relaying Wnt/β-catenin to pre-B cell receptor signaling pathways through the regulation of Runx/CBFβ heterodimerization was verified by employing niclosamide, XAV939, and LiCl as Wnt inhibitors and activator, respectively. Expand
A Major Deletion in the Vκ–Jκ Intervening Region Results in Hyperelevated Transcription of Proximal Vκ Genes and a Severely Restricted Repertoire
These findings reveal previously unrecognized additional functions for cis-elements within the Vκ–Jκ intervening region, namely, prevention of the production of massive levels of noncoding RNA species by silencing transcription of germline proximal Vκ genes in both developing and mature B cells. Expand
Crlz1 activates transcription by mobilizing cytoplasmic CBFbeta into the nucleus.
In coimmunop Recipitation and chromatin immunoprecipitation experiments, Crlz1 was found to be bound to the resulting CBF heterodimer in a form of ternary complex and to remain in that ternARY complex even when CBF bound to its target DNA site such as IgJ enhancer. Expand
The promoter of the Immunoglobulin J Chain gene receives its authentic enhancer activity through the abutting MEF2 and PU.1 sites in a DNA-looping interaction.
A DNA-looping interaction between the IgJ promoter and its enhancer was demonstrated using chromosome conformation capture assays not only in IgJ-expressing S194 plasma cells but also during interleukin-2-induced BCL1 B-cell terminal differentiation. Expand
Stage-Specific Expression of Two Neighboring Crlz1 and IgJ Genes during B Cell Development Is Regulated by Their Chromatin Accessibility and Histone Acetylation1
The IgJ gene is expressed in the plasma cell stage. However, its neighboring charged amino acid-rich leucine zipper 1 (Crlz1) gene, which is mapped 30 kb upstream of the IgJ gene in mice, is shown toExpand
Balance between MAT2A intron detention and splicing is determined cotranscriptionally.
A model in which the MAT2A-DI transcript is not a precursor to mRNA but is instead a "dead-end" RNA fated for nuclear decay is supported, and it is concluded that polyadenylated RNAs with DIs can have at least two distinct fates. Expand
The HSS3/4 enhancer of Crlz1-IgJ locus is another target of EBF in the pre-B cell stage of B cell development.
It is shown that the protein causing the pre-B cell-specific in vivo footprint on a sequence similar to the EBF-binding consensus is truly EBF as judged by EMSA using various oligo-DNA competitors and anti-EBF antibodies. Expand