Bottom-up fabrication of nanoscale structures relies on chemical processes to direct self-assembly. The complexity, precision, and yield achievable by a one-pot reaction are limited by our ability to encode assembly instructions into the molecules themselves. Nucleic acids provide a platform for investigating these issues, as molecular structure and… (More)
Fig. S1. Cross-DNA motif, tile A: Schematics of strand structures and DNA sequences. Tile A consists of nine different strands indicated by different colors. The red-dot on the A9 strand indicates the site of biotin modification for demonstration of addressability. Arrows in drawings indicate strand direction running from 5' to 3'.
Notes Nucleation Since in the range of conditions we are considering, tile attachment by a single sticky end is thermodynamically unfavorable, while attachment by two sticky end bonds is favorable, there is a kinetic barrier to the formation of assemblies if all possible assembly paths involve multiple unfavorable tile attachment steps. The critical nucleus… (More)
Controlled mechanical movement in molecular scale devices is one of the key goals of nanotechnology. DNA is an excellent candidate for the construction of such devices due to the specificity of base pairing and its robust physicochemical properties. Well-known as the genetic material, DNA has recently been explored as a smart material for constructing… (More)
Synthesizing molecular tubes with monodisperse, programmable circumferences is an important goal shared by nanotechnology, materials science, and supermolecular chemistry. We program molecular tube circumferences by specifying the complementarity relationships between modular domains in a 42-base single-stranded DNA motif. Single-step annealing results in… (More)
We demonstrate the precise control of periodic spacing between individual protein molecules by programming the self-assembly of DNA tile templates. In particular, we report the application of two self-assembled periodic DNA structures, two-dimensional nanogrids, and one-dimensional nanotrack, as template for programmable self-assembly of streptavidin… (More)
DNA-based nanotechnology is currently being developed as a general assembly method for nanopatterned materials that may find use in electronics, sensors, medicine, and many other fields. Here we present results on the construction and characterization of DNA nanotubes, a self-assembling superstructure composed of DNA tiles. Triple-crossover tiles modified… (More)
— We have designed and experimentally demonstrated the self-assembly of an addressable DNA lattice (i.e., a unique tile for each position in the lattice) using a two-step tile annealing procedure. Our method can be applied to a variety of systems including algorithmic tiling systems to expand the basic tile set.
We report on the self-assembly of one-and two-dimensional DNA scaffolds, which serve as templates for the targeted deposition of ordered nanoparticles and molecular arrays. The DNA nanostructures are easy to reprogram, and we demonstrate two distinct conformations: sheets and tubes. The DNA tubes and individual DNA molecules are metallized in solution to… (More)
We present a DNA nanostructure, the three-helix bundle (3HB), which consists of three double helical DNA domains connected by six immobile crossover junctions such that the helix axes are not coplanar. The 3HB motif presents a triangular cross-section with one helix lying in the groove formed by the other two. By differential programming of sticky-ends, 3HB… (More)