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A monoclonal antibody (Mab J1-31) has been produced by using human brain homogenate as immunogen in mouse. Double-label immunofluorescence microscopy on cryostat sections of human, rabbit and rat brain, reveals staining of cells that are also stained with antiserum to glial fibrillary acidic protein (GFAP, a commonly used marker protein for astrocytes).(More)
The extensor digitorum longus muscles of rat were stained for the localization of acetylcholinesterase activity at the neuromuscular junctions. The modified methods of Koelle-Friedenwald and Karnovsky-Roots were used with acetylthiocholine iodide as the substrate. The merits and demerits of both these methods are discussed. TEM and SEM X-ray dispersive(More)
A mouse monoclonal antibody (MAb J1-31, isotype IgG 2b) was raised against an autopsy sample of cerebral white matter from a multiple sclerosis (MS) patient. MAb J1-31 recognizes a protein (J1-31 antigen) in human brain which has a molecular weight of approximately 30,000 daltons (30 kD) as determined by immunoprecipitation followed by SDS-gel(More)
The structure of gap junctions in unfixed and glutaraldehyde fixed rapid-frozen mouse liver has been examined in the rotary shadowed freeze-fracture replicas. Glutaraldehyde fixation alters the packing of connexons and reduces the average interconnexon spacing from 10.2 +/- 2.4 nm in unfixed preparations to 8.7 +/- 1.9 nm in the fixed preparations. The(More)
The astroglial reaction following a laceration-type surgical lesion of rat spinal cord is recognized by hypertrophy of astrocytes. This phenomenon can be readily demonstrated by enhanced immunoreactivity for glial fibrillary acidic protein (GFAP) and a recently discovered 30 kD protein (J1-31 antigen). The results reported in this article lead to the(More)
X-ray energy dispersive microanalysis of the reaction product in Karnovsky and Roots histochemical localization of acetylcholinesterase indicated the presence of sulfur, iodine, copper, and iron. The reaction was run in vitro using purified acetylcholinesterase from the electric eel to confirm our previous results on similarly treated neuromuscular junction(More)
Mouse liver gap junctions have been isolated by using an anionic detergent, n-dodecanoyl sarcosine, in combination with non-ionic polyoxyethylene ethers (Brij 35 and Brij 58) and (W-1) detergents. Purified gap junctions are obtained in a sucrose step gradient containing 1-o-n-octyl-beta-D-glucopyranoside. This procedure is aimed at minimizing proteolysis.(More)
Effect of cyclic AMP (cAMP) on Phycomyces blakesleeanus was studied by growing sporangiospores on glucose-asparagine agar or liquid medium containing three different levels of cAMP (10, 20 and 40 micronM) in addition to the control (no cAMP added). The response of Phycomyces to the exogenous cAMP concentration in the medium is as follows: (1) the time(More)
This review provides an historical and current perspective on astrocytes in the CNS, detailing their morphological and biochemical diversity, origins and transformation to reactive astrocytes in experimental lesions and clinical disease states which result in gliosis. Recent studies on reactive astrocytes have been facilitated by the availability of(More)