• Publications
  • Influence
Avidin Expressed in Transgenic Rice Confers Resistance to the Stored-Product Insect Pests Tribolium confusum and Sitotroga cerealella
Rice (Oryza sativa var. Nipponbare) was transformed with an artificial avidin gene. The features of this construct are as follows: (1) a signal peptide sequence derived from barley alpha amylase wasExpand
  • 31
  • 5
  • Open Access
Molecular Cloning and Functional Expression of cDNA Encoding a Cysteine Proteinase Inhibitor, Cystatin, from Job's Tears (Coix lacryma-jobi L. var. Ma-yuen Stapf)
A λZAP II cDNA library was constructed from mRNA in immature seeds of the grass Job's tears. A cDNA clone for a cysteine proteinase inhibitor, cystatin, was isolated from the library. The cDNA cloneExpand
  • 19
  • 2
Cultivar identification of rice (Oryza sativa L.) by polymerase chain reaction method and its application to processed rice products.
As the cultivars of rice markedly affect eating quality, processing suitability, and price, identification or differentiation of rice cultivar is very important. We developed suitable 14 STSExpand
  • 33
  • 1
Differentiation and search for palatability-factors of world-wide rice grains by PCR method
米の食味に品種が強く影響することはよく知られているが,品種特性はDNAによって規定される.そこで,本研究では,これまでに蓄積した品種判別用の各種プライマーおよび新たに開発したデンプン特性やタンパク質組成に関係するプライマーを用いるPCRを利用したDNA食味推定技術の開発を目的に研究を行い,以下の結果を得た.Expand
  • 13
  • 1
  • Open Access
Novel preparation method of template DNAs from wine for PCR to differentiate grape (Vitis vinifera L.) cultivar.
Template DNAs were extracted from wine and purified for use as samples for PCR to differentiate grape cultivars. It has been pointed out that the authentication of grape material by PCR using wine asExpand
  • 28
  • 1
Identification of domestic glutinous rice cultivars by the PCR method using grains of 18 typical glutinous rice cultivars as sample and development of technology for detection of different kind grain
糯加工品において,ワキシーコーンなどの異種穀類を検出する技術の開発が,食品表示への信頼の向上と,品質の向上に必要と考えられ,異種穀類(トウモロコシ,大麦)検出のためのプライマーの開発を行いPCR法でこれらの穀類の検出を可能とした.また糯加工品であるあられは,餅の製造工程に,「焼き」の工程が入りDNA抽出が困難とされていたが,酵素法を改良することにより純度の高いDNAが得られ,Expand
  • 4
  • 1
Soluble starch synthase I effects differences in amylopectin structure between indica and japonica rice varieties.
The effect of soluble starch synthase I (SSI) on differences of amylopectin structure between the indica and japonica rice varieties was investigated. Native-PAGE/active staining analysis showed thatExpand
  • 17
  • 1
Quality Evaluation of Rice Crackers Based on Physicochemical Measurements
The processing suitability as a material for rice crackers was characterized in the present study, based on physicochemical measurements and sensory testing of high-quality premium rice, low-amyloseExpand
  • 13
  • 1