Learn More
Binding of regulatory proteins to multipartite DNA binding sites often occurs with protein-protein interaction, resulting in cooperative binding. The operators of bacteriophage lambda have several pairs of repressor binding sites (O(R)1-O(R)2, O(R)2-O(R)3, O(L)1-O(L)2, and O(L)2-O(L)3) separated by a variable number of base pairs, and thus, bacteriophage(More)
We present a hydrogen sensor based on metallic photonic crystal slabs. Tungsten trioxide (WO(3)) is used as a waveguide layer below an array of gold nanowires. Hydrogen exposure influences the optical properties of this photonic crystal arrangement by gasochromic mechanisms, where the photonic crystal geometry leads to sharp spectral resonances.(More)
A lambda-repressor mutant, S228N, which is defective in tetramer formation in the free state but retains full cooperativity, was studied in detail. Isolated single operator-bound S228N repressor shows association properties similar to those of the wild-type repressor. Fluorescence anisotropy studies with dansyl chloride-labeled repressor show a(More)
A non-cooperative mutant of lambda-repressor, Y210C, has been purified and characterized. The mutant protein does not show any evidence of cooperative interaction as judged by difference near-UV circular dichroism spectra of DNA. The mutant protein also shows much weaker self-assembly as revealed by fluorescence anisotropy measurement. The far-UV circular(More)
There are three recognized low-loss configurations for waveguide laser resonators in which the waveguide is either closed at each end by a plane mirror (dual case I design) or one of the plane mirrors is replaced by a curved mirror at some distance from the guide exit. Some time ago, a variant of the latter design was proposed by exploiting the self-imaging(More)
  • 1