Subimal Basu

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Stress activates the hypothalamic-pituitary-adrenal axis through release of corticotropin releasing hormone (CRH), leading to production of glucocorticoids that down-regulate immune responses. Acute stress, however, also has proinflammatory effects that seem to be mediated through the activation of mast cells. Stress and mast cells have been implicated in(More)
We demonstrate an alternative method for the generation of random subclones for large-scale shotgun human DNA sequencing projects. Miniprep DNA from a human cosmid clone was partially digested with CviJI, size-fractionated by agarose gel electrophoresis and cloned into bacteriophage M13. A library consisting of 10(5) subclones of 1.1 kb average size was(More)
BACKGROUND Mast cells are involved in allergic inflammation by secreting histamine, proteases and several cytokines, including interleukin (IL)-6, tumor necrosis factor-alpha (TNF-alpha) and IL-8. Certain histamine-1 receptor antagonists, such as azelastine present in the ophthalmic solution Optivar, have been reported to inhibit histamine and tryptase(More)
Stress activates the hypothalamic-pituitary-adrenal (HPA) axis through release of corticotropin releasing factor (CRF), leading to production of glucocorticoids that down regulate immune responses. However, acute stress via CRF also has pro-inflammatory effects. We previously showed that acute stress increases rat blood-brain barrier (BBB) permeability, an(More)
The gastrokinetic effects of hot water extract of black tea [Camellia sinensis, (L) O. Kuntze (Theaceae)] on gastrointestinal motility were studied both in vivo and in vitro. The extract significantly accelerated the gastrointestinal transit (GIT) in vivo in mice. These facilitatory effect was reduced after pretreatment with atropine, hemicholinium-3,(More)
The effect of various fractions of black tea [(Camellia Sinensis) (L) O. Kuntze (Theaceae)] on the function of mammalian skeletomotor apparatus was studied. The theaflavin fraction (Tfs) produced a concentration- dependent facilitation of indirect twitch responses of the rat phrenic nerve diaphragm preparation and the facilitation was dependent on the(More)
Increased oxidative stress contributes to chronic neurodegenerative diseases, yet the underlying mechanisms are poorly understood. Hippocampal slice cultures prepared from 20-30-day-old mice or rats were used to model chronic neuronal loss following oxidative stress. Neuronal loss was initiated by inhibition of the antioxidant enzyme, superoxide dismutase(More)
S. Martin, S. Basu, D. Schaafsma, A.J. Halayko, Department of Physiology, Faculty of Medicine, University of Manitoba and Manitoba Institute of Child Health, Winnipeg, MB Caveolin-1 (Cav-1) can modulate intracellular signaling pathways in airway smooth muscle (ASM) that may mediate inflammation, contraction, and/or proliferation. Previously, we observed(More)