Subhendu Ghosh

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Voltage-dependent anion channel (VDAC) is reported to be the receptor for plasminogen kringle5. In this paper, the interaction of VDAC from rat brain mitochondria with plasminogen protein has been investigated through bilayer electrophysiological studies. We report for the first time that interaction of plasminogen with VDAC leads to partial closure of the(More)
Voltage-dependent anion channel (VDAC), Bax, and tBid play a central role in apoptosis regulation but their functioning is still very controversial. VDAC forms voltage gated pore in planar lipid bilayers, and acts as the pathway for the movement of substances in and out of the mitochondria by passive diffusion. Here we report that there is increase in the(More)
– Noise profile of Voltage Dependent Anion Channel (VDAC) is investigated in open channel state. Single-channel currents through VDAC from mitochondria of rat brain reconstituted into a planar lipid bilayer are recorded under different voltage clamped conditions across the membrane. Power spectrum analysis of current indicates power law noise of 1/f nature.(More)
Apoptosis is a controlled form of cell death that participates in development, elimination of damaged cells and maintenance of cell homeostasis. Also, it plays a role in neurodegenerative disorders like Alzheimer's disease. Recently, mitochondria have emerged as being pivotal in controlling apoptosis. They house a number of apoptogenic molecules, such as(More)
Collective behaviour of a crown ether channel, bis[(benzo-15-crown-5)-15- yl methyl] pimelate, in a planar lipid bilayer membrane has been studied through electrophysiological methods. A characteristic feature of these channels is their sequential opening, indicated by a uniform stepwise increase in the multi-channel current. The experimental results show(More)
Gap junction provides low resistance pathways for cell-to-cell passive diffusion of ions, metabolites, second messengers etc. and thus, controls development, differentiation in embryonic tissues, and communication in adult tissues. It has been pointed out in our previous work that these passive diffusion channels behave cooperatively which in turn depends(More)
KvAP is a voltage-gated tetrameric K(+) channel with six transmembrane (S1-S6) segments in each monomer from the archaeon Aeropyrum pernix. The objective of the present investigation was to understand the plausible role of the S6 segment, which has been proposed to form the inner lining of the pore, in the membrane assembly and functional properties of KvAP(More)
Stress activated c-Jun N-terminal Kinase-3 (JNK3) has been reported to act on mitochondrion to promote neuronal cell death. Phosphorylation of mitochondrial Voltage-Dependent Anion Channel (VDAC) plays an important role in mitochondria-mediated cell death. Keeping these in view phosphorylation of rat brain VDAC by JNK3 has been studied in vitro. Pro Q(More)
Photodissociation dynamics of propyne at 193 nm are studied using the fewest switches nonadiabatic trajectory surface hopping method on its first excited singlet electronic state (1(1)A''). The trajectories are propagated based on potential energies, gradients and nonadiabatic couplings calculated at the MRCIS(6,7) level with the 6-31++G(d,p) basis set. Our(More)
Bax and Bax interacting factor-1(Bif-1) are cytosolic proteins, which translocate towards mitochondria during mitochondria-mediated apoptosis. Bif-1 has been identified to co-immunoprecipitate with Bax in apoptotic cells. We have studied the interaction of Bax and Bif-1 on Bilayer Lipid Membrane (BLM) through electrophysiological experiments. It has been(More)