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Violacein (Vio) is an important purple pigment with many potential bioactivities. Deoxyviolacein, a structural analog of Vio, is always synthesized in low concentrations with Vio in wild-type bacteria. Due to deoxyviolacein’s low production and difficulties in isolation and purification, little has been learned regarding its function and potential(More)
In order to process the fuzzy and imprecise information in the evidential reasoning, the scholars have made many attempts to generalize belief and plausibility functions based on the Dempster-Shafer(D-S) evidence theory to fuzzy sets for many decades. A new method for defining the fuzzy closeness degree is put forward in this paper. Based on the closeness(More)
Nowadays, baculovirus-infected insect cells and tetracycline-inducible mammalian cell lines (T-REx-293) are intensively used for G protein-coupled receptor (GPCR) production for crystallography purposes. Here we constructed a suspension T-REx-293 cell line to stably express an engineered neurotensin receptor 1 (NTS1) mutant and we quantitatively compared(More)
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