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OBJECTIVE To construct an stx2 gene mutant phage phi Min27(delta stx::cat) and to observe its infectiousness of various serotypes Escherichia coli strains. METHODS With the help of Red recombinant system, the stx2 gene of the E. coli O157:H7 Min27 strain isolated from intestinal feces of piglet with diarrhea at a swine farm of Shanghai, was replaced by(More)
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